No. 6. DEPARTMENT OF AGRICULTURE. Ill 



Sydney Maitin ftnl hiuiian tuberculosis spiitiuii to six calves. 

 Four of these received each 70 c.c. mixed with rlieir food at one meal. 

 They were killed after 3?., 03, 8.1. and 28.5 days. The first three 

 showed respectively 53, 63 and 13 tubercular nodulesiu the intestine, 

 while the fourth was entirely free. The two other calves were given 

 440 c.c. of sputum at one feeding. One was killed after fifty-six days, 

 and showed tuberculosis of the intestine and mesenteric gland. The 

 other was allowed to live 1.3S days, and was free from disease when 

 killed. 



At the recent Congress on Tuberculosis. Prof. Thomassen. of 

 Utrecht, reported the following experiment: A calf four weeks old 

 was inoculated in the anterior chamber of the eye with a pure cul- 

 ture of the tubercle bacillus isolated from a ease of tuberculous 

 arthritis in man. An intense keratitis was s^t np. the cornea becom- 

 ing so opaque tliat it was impossible to observe the alterations in 

 the iris. It was killed after six weeks, and was found to be the 

 victim of a pretty well generalized tuberculosic?. Both lungs con- 

 tained numerous miliary tubercles and some gray fibrous tubercles 

 of larger size. The path of infection from the eye to the lung was 

 mapped out by the condition of the subparotideal, cervical, medias- 

 tinal, and bronchial lymph glands of the same side. 



Xocard states that by inocnlation into the aiadinoid cavity of 

 a small amount of liuman nilirrcle bacilli a rajiidly fatal tubercular 

 meningitis is set up identical with that seen in children. A calf, five 

 months old was thus inoculated by him on August 2. 1001. and died 

 OR the 28th of the same month, the pia mater being infiltrated with 

 tubercles wihich proved virulent for guinea pigs. 



On the same day Xocard injected into the jugular of a cow which 

 had become much reduced by chronic diarrhea, but which was free 

 from tuberculosis as shown by the tuberculin test. T c.c. of a suspen- 

 sion of hnnian bar-illi. She died September Irli. and showed a num- 

 ber of small foci, softened or caseous and extremely rich in tubercle 

 bacilli. He considers that in this case tihe animal was so weakened 

 by the pre-existing diarrhea that its normal resistance was largely 

 destroyed. 



De Jong as the result of a series of comparative inoculations of 

 human and bovine tubercle bacilli, states that seven cattle, namely, 

 two calves six months old. three steers two years old and one eigh- 

 teen months, and one calf seven or eight months old. all became 

 tuberculous by the injection of pure cultures of the human tubercle 

 bacillus. In only one of the seven was the disease grave and wide- 

 spread; in four the lesions were retrogressive, and in the two remain- 

 ing ones progressive. 



Arloing reports the following recent experiments: Three cultures 

 of human origin were used, one of which had been under cultivation 



