No. 6. DEPARTMENT OF AGRICULTURE. 113 



illustrated in the specimens presented here for your inspection and 

 study. I cannot but feel that these specimens possess an unusual 

 interest and importance in the solution of a problem which is now 

 engaging- the attention of scientific men throughout tihe world, and 

 which has been made the subject of special investigation by the gov- 

 ernments of many countries. Our first successful attempt to infect 

 cattle with human tuberculosis was made in 1898, when four calves 

 of nearly the same age, four to five weeks old, received intraperi- 

 toneally 10 c.c. of human tuberculous sputum, from different sources, 

 but in all cases containing a large number of bacilli. One showed 

 no ill effects except a ©light rise in temperature, and the autopsy 

 was entirely negative. 



Of the other three, two (had persistent high temperature following 

 the injection, but only one showed marked illness otherwise. Post- 

 mortem examination proved that all had become infected with tuber- 

 culosis, the lesions in two being quite extensive. I will give here the 

 details of onlj^ the one which showed marked illness during life. 

 Ihe lesions were so extensive and typical that even the most skepti- 

 cal must admit tihe success of the experiment, and I have here some 

 of the organs for inspection. 



Calf No. 8050, aged four weeks, wasi inoculated on May 16, 1898, 

 with sputum from an early case of pulmonary tuberculosis at the 

 University Hospital. The sputum contained a large number of tu- 

 bercle bacilli. Soon after the operation the temperature of the calf 

 rose and continued high, with some remissions, until it was killed. 

 Its appearance was bad, the coat dry and rough, the respiration 

 rapid. It was tested with tuberculin, but the temperature was too 

 high for results. It was killed on August 1st, weighing 190 pounds. 

 On the surafce of botlh lungs there was a slight deposit of fibrin, and 

 on section a number of hemorrhagic areas were observed in both. 

 The mediastinal and bronchial glands were enlarged and congested. 

 The abdominal cavity contained about 12 ounces of bloody serum. 

 The peritoneum was thickly studded over its entire surface with 

 nodules from 1 mm. to 12 mm. in diameter, fibrous in character. In 

 many places these nodules had massed together, forming tumors, 

 some 5 cm. in diameter, which were dense and fibrous. The spleen 

 contained many nodules, both on its surface and througihout its sub- 

 stance. The whole omentum was thickly studded with nodules from 

 2 mm. to 12 mm. in diameter, and besides which there were three 

 large masses, dense and fibrous in character, two of which were 15 

 cm. long by 7 cm. wide, and 12 mm. thick; and the third 7 cm. long 

 by 6 cm. wide, by 4 cm. thick. The abdominal surface of the dia- 

 piiragm was thickly studded with nodules, fibrous in character. The 



8—6—1902 



