No. 6. DEPARTMENT OF AGRICULTURE. 115 



jugular vein. On the next day the temperature had risen to 108.2 F. 

 After a slijj;ht remisssion it rose to 103 degrees P. on the seventh day 

 after inoculation, and continued to rise each day, reaching 10(5.4 de- 

 grees F. by the fourteenth day, after wlhich it fell slightly until death 

 which took place on December 21st, seventeen days after inoculation. 

 The animal showed marked illness during life, the respiration reach- 

 ing 08 per minute. 



Autopsy. — The lungs and the related glands showed macroscopi- 

 eally the most marked disease. Both lungs were thickly studded 

 throughout, with remarkable uniformity, with miliary nodules giv- 

 ing a sandy feel under the linger. Tlie left was pneumonic, owing to 

 the position of the calf for some time before death. Portions of the 

 right were in a similar condition, sinking when placed in water. 

 The bronchial and mediastinal glands were much enlarged and soft, 

 and in the former small areas of caseation were found. 



The liver was large, soft, and friable. Several nodules were visi- 

 ble on the surface, though not perceptible to the touch. The spleen 

 showed very sligdit changes. The kidneys were normal. 



Calf No. 265D7, weight 202 pounds. Tested with tuberculin, and 

 gave no reaction. On December 4, 1901, 5 c.c. of a suspension of 

 Culture BB of the fifth and Sixth generations were injected into the 

 peritoneal cavity. The temperature rose to 105.2 degrees F. by the 

 eighth day after injection, remaining very nearly the same until four 

 days before death, when it began to fall, and reached 103 degrees F. 

 on the diay before death, which took place on December 31st, or 

 twenty-seven days from the time of inoculation. 



Autopsy. — Great emaciation. Loss in. weight thirty-seven pounds. 

 The lungs were largely involved, though not uniformly. The cen- 

 tral and posterior portions showed many areas deep red in color and 

 solid to the feel. These were thickly studded throughout with min- 

 ute grayish nodules. 



The suprasternal lymph glands were as large as goose eggs, and 

 contained cheesy areas. The mediastinal glands were much en- 

 larged, one being six inches in length by two inches in diameter. 

 The peritoneal cavity contained about six quarts of straw-colored 

 fluid. The peritoneum was everywhere enormously thickened, and 

 practically converted into a tuberculous mass. In several places 

 it was one-half an inch thick and fibrous. The omentum was also 

 enormously thickened and bound over the surface of the liver. It 

 contained a great number of nodules, dense and fibrous, many with 

 caseous centers. The liver was firmly adherent to the diaphragm, 

 and on section showed many nodules. The spleen was firmly at- 

 tached to the surrounding parts by a mass of partly organized fibrin, 

 which contained many cheesy nodules. On section it appeared to be 

 normal. 



