116 ANNUAL REPORT OF THE Off. Doc. 



Cow No. 45030, about three years old, and weighing GGO poimdis. 

 On January 22, 11)02, 2^ c.c. of a suspension of Culture BB of the 

 seventh and eighth generations were injected into the rigiht jugular 

 vein, and the eanie amount into the peritoneal cavity. The tempera- 

 ture rose to 108.2 degrees F. on the second day, then fell slightly 

 during the next six days, after which it rose steadily, reaching the 

 maxiinum, 107 degrees F., three days before death, after which it fell 

 sligiJitly, the animal dying seventeen days after inoculation. The 

 lungs were mostly involved, both being thickly studded throughout 

 with miliary nodules. Most of the left lung showed interlobular 

 emphysema, while the inferior portion was much congested. On 

 the omentum were numerous dark red spots which could be felt be- 

 tween the fingers. The spleen was adherent to the diaphragm, and 

 ttliere were many punctate hemorrhages on the surface. The liver 

 and kidneys showed no gross changes. 



What interpretation w to be given to these remarkable results? 

 One of two propositions must be admitted: either we have found a 

 human tubercle bacillus having a pathogenic power for cattle quite 

 as great as any bovine germ, or else we have found in the mesienteric 

 gland of a child the bovine tubercle bacillus. If we accept the law 

 of diagnosis laid down by Koch, namely, the inoculation test, the 

 latter is the true explanation. I, myself, am strongly of the opinion 

 that this latter is the case for the following reasons: 



1. The history of the case as given by Dr. Hand of primary intes- 

 tinal infection. 



2. Morphologically and culturally the organism corresponds more 

 nearly to the bovine type as first defined by Dr. Theobald Smith, and 

 later by ourselves, than the human. 



3. The great pathogenic power for cattle, 



A second culture obtained from the mesenteric glands of a child 

 has ehown a virulence far in excess of that usually found in human 

 cultures, tihrough it falls short of Culture BB in pathogenic power. 

 This culture, designated U, was isolated in December, 1900, and 

 tested for guinea-pigs and rabbits. The results, together with its 

 manner of giowing and microscopic appearance, lead us to consider 

 it a typical human culture. During the summer of 11)01 it was se- 

 lected for some feeding experiments on puppies, and showed a de- 

 gree of virulence that was not expected. In December last, wishing 

 to produce a slow tuberculosis in a dog, this culture was again em- 

 ployed. The dog grew ill rapidil^', and died in January, thirty-six 

 days from the time of inoculation. Post-mortem examination re- 

 vealed a typical and extensive tuberculosis and it was decided to 

 inoculate a calf in order to test its virulence for the bovine race. On 

 March 4, 1902, Calf No. A 4.5046, weighing 74 pounds, was inoculated 

 in the jugular vein with 6 c.c. of a suspension of Culture U, of the 



