No. 6. DEPARTMENT OF AGRICULTURE. 119 



Amount of Length of 



No. Injection life. 



1. Calf 26562 925 c.c. 106 days 



2. Calf A45020 16 c.c. 48 days 



3. Calf AJ5035 13 c.c. 23 days 



4. Calf A35047 10 c.c. 24 daya 



5. Calf A45073, 14 c.c. ai daya 



Calf No. 26502, weight 210 j)oimds. The lirst inoculation vva« mad«.' 

 on September (>, 1001, and thereafter at intervals of a week, until 

 December KUh. Each time tilie total amount was divided into four 

 equal parts and Injected subcutaneously, intraperitoneally, into the 

 lung, and into the jugular vein. The commencing dose was 10 c.c. 

 of an emulsion ecjnal in opacity to a twenty-four-hour-old bouiUon 

 culture of the tyi)hoid bacillus, the total amount given being 925 c.c. 

 The temperature rose after each injection, and remained high for 

 two days, then fell gradually, though normal was never reached. 

 About five weeks after the first injection the calf began to cougih. 

 This cough grew steadily wor^e until death, being most marked on 

 the day following each injection. Respiration grew labored, and 

 the animal lost flesh. By December 15th it was markedly ill. Death 

 took place during the night of December 21st. 



Autopsy. — Considerable emaciation. The lungs were hard and 

 firm to the touch. The various sites of inoculation could not be de- 

 tected, and there were no abscess cavities nor seal's. Tllie lungs were 

 dense throughout and heavy, through sections floated in water. The 

 bronchi, especially the smaller ones, were filled with a tenacious 

 mucus, in which many tubercle bacilli were found. An emulsion 

 made from one of them showed many tubercle bacilli. The medias- 

 tinal glands w^ere large and soggy, but no nodules could be found. 

 The bronchial glands were enlarged, and a few caseous points were 

 found. An emulsion made, from one of them showed many tubercle 

 bacilli. Tihe mediastinal glands were large and soggy, but no 

 nodules could be found. The liver was attached to its capsule at 

 points which were the sites of flat, yellowish nodailes, from 2 mm. to 

 5 mm. in diameter. On section tuberculous areas of considerable 

 extent were for.nd, reaching from the surface to a depth of 3 cm. 

 TTie spleen was much enlarged and very soft. On the surface were 

 a number of flat fibrous new-growths. The kidnevs showed no 

 changes. The omentum was much discolored, having a dirty, brown- 

 islli appearance, and in parts much thickened. It contained upward 

 of 100 nodules of 2 mm. to 6 mm. in diameter, some gray, some white, 

 and some hemorrhagic. Some had the appearance of beginning 

 "grape'' formation. The mesentery presented the same appearance. 

 Its glands were enlarged and wet, but no cheesy nodules could be 

 found. On the parietaJ peritoneum were a large number of pearly 



