120 ANNUAL REPORT OF THE Off. Doc. 



nodules, 2 mm. in diameter, and a few sessile new-growths, some of 

 which wen hemorrhagic, and had fringes of fibrin hanging from 

 them. Microscopic examination of the tissues showed tihe spleen 

 and kidneys to be free of tubercles. In the lungs the greater part 

 of both is made up of conglomerated tubercles which show very little 

 central caseation. The alveoli are completely filled with desqua- 

 mated epithelium, leukocytes, and epitheliod cells. There is a 

 marked bronchitis. 



Calf No. 26503, weight 1G5 pounds. The first inoculation was 

 made on September n, 1901, and thereafter weekly until December 

 16, 1901, when an interval of nineteen days was allowed to elapse, 

 the inoculations being resumed on January 4, 1902. The method of 

 inoculation was the same as for Calf No. 26562. Death took place 

 January 22, 1902, the total amount of the suspension of the culture 

 given being 1400 c.c. The condition of the animal following the in- 

 jections correspond closely with Calf No. 26562, its survival being 

 somewhat more prolonged. 



Autopsy. — Weight 186 pounds. Considerable emaciation. The 

 lungs were firm and non-elastic to the touch. TTie points of inocu- 

 lation could not be detected either in the pleurae or in the lungs, ex- 

 cept at one point, where a small pus cavity, 1 cm., was found near 

 tihe surface of the right lung, the pus containing many tubercle 

 bacilli. No district nodules could be found in either lung, but both 

 were much increai&ed in solidity throughout through sections would 

 float in water. The bronchi were filled with a tenacious mucus. The 

 pleurae were roughened by bands of fibrin, which extended to the 

 lung, attaching the inferior borders especially to the dhest-wall. 

 The bronchial and mediastinal glands were much enlarged, and the 

 latter contained many caseous nodules. In the abdominal cavity 

 all the organs were attached to the peritoneum and to each other by 

 bands of fibrin. The liver was firmly adherent to the diaphragm. 

 Over the surface were fifty to sixty \Mhitish, flat nodules, 5 mm. to 100 

 mm. in diameter some of them extending deeply into the substance 

 of the organ. On section caseous areas were seen. 



The spleen and kidneys showed slight changes only. The parietal 

 peritoneum was rough at many points and had fringes of fibrin at- 

 tached to it. Scattered over it were a large number of gray nodules, 

 2 mm. to 5 mm. in diameter. 



Microscopic examination showed no well-defined tubercles in the 

 spleen and kidneys. The liver showed many areas of necrosis. In 

 the lungs were masses of conglomerated nodules, most of whicih did 

 not show marked central caseation. Numerous giant cells were 

 found. The alveoli are filled with desquamated epithelium, leuko- 

 cytes, and epithelioid cells. There is a marked bronchitis. 



