No. 6. DEPARTMENT OF AGRICULTURE. 121 



Calf No. 4.5020, six months old. On December 23, 1901, 8 c.c. of 

 an emulsion made from the bronchial gland of Calf No. 20562 were 

 injected into the left lung, and the same amount into the peritoneal 

 cavity. The emulsion showed many tubercle bacilli. I5y January 

 15, 1!)02, the calf was markedly ill, and breathing was increased in 

 rapidity. Those symptoms increased steadily, the temperature rang- 

 ing from 101 degrees F. to 105.5 degrees F. About February 1st the 

 temperature began to fall, and at the time of death, February 9th, 

 was subnormal. 



Autopsy. — Much emaciated. Both lungs were a dark red, and 

 were thickly ©own throughout with miliary nodules. The left lung 

 was adherent to the chest-wall over a large part of its surface. On 

 separating it many tubercular new-growths were seen, between 

 wihich the pleura was smooth. At the point of inoculation was an. 

 abscess cavity 4 cm. in diameter. The pus contained many tubercle 

 bacilli and staphjiococci. On the lung over the abscess, and bind- 

 ing it liiihtlv to the che^j^t-wall, was a laver of fibrin, of cream- white 

 color. Along the backbone was a chain of enormously enlarged 

 glands fitting into the spaces between the ribs, and extending from 

 the first rib to the diaphragm. These were filled with caseous areas. 

 The mediastinal glands were enlarged and showed many caseous 

 areas. Most striking was the condition of the omentum, which had 

 been converted into a mass of tubercular new-growth, made up of 

 conglomorate nodules. It varies in thickness from 1 cm. to 3 cm. 

 The nodules are grayish-yellow, fibrous, and show central caseation. 

 The liver sl-ows numerous yellowish nodlues, both on the surface and 

 on section. 



The spleen has on its surface a number of flat, fibrous news- 

 growths, but no nodules are seen on section. 



The kidneys are normal in appearance, but a few nodules appear 

 on section. 



Microscopic examirkation shows the lungs to be thickly sown 

 with minute tubercles. Sections stained with carbol-fuchsin show 

 innumerable tubercle bacilli clustered mainly in these nodules. 

 The liver contains manv necrotic areas in which ma«v tubercle 

 bacilli are found. The spleen contains a considerable number of 

 necrotic areas. The flat new-growths are tuberculosis. The kid- 

 neys show only a few tubercles, which contain innumerable tubercle 

 bacilli. 



Cfilf No. 45035, aged nine weeks, weight 130 pounds. February 

 11, 1902, inoculated into its right lung and peritoneal cavity with an 

 emulsion made from <he omentum of Calf No. 45020, 5 c. c. being in- 

 jected into the lung, and 8 c. c. into the peritoneal cavity. The tem- 

 perature rose on the day after the inoculation, and reached 107 de- 



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