144 ANNUAL REPORT OF THE Off. I>oc. 



Inn^ and in the broncliial and uuMliastiiial lymphatic jjlands, the post- 

 pharyngeal a^)d intoi-niaxillary lymphatic <;lands and in the mesen- 

 teric lynij)hatic "ilands. The lesions in this control cow were more 

 widely distribnted a«d more advanced than in the cow that had re- 

 ceived hirge quantities of tuberculin. 



The other two cows of the experiment were killed December 16, 

 3901. In the first of thot'o (26557) which liad received the injections 

 of tuberculin, no lesions of tuberculosis were found exceptinji in the 

 mesenteric lymphatic jilands. A few of these jjlands of both the 

 small and large intestine showed small areas of caseation. The 

 second control cow (26556) showed lesions of tuberculosis in both 

 lunos, the bronchial, mediastinal and postpharyngeal glands; and 

 (he lympatic glands of the mesentery were more extensively involved 

 thao in tlie preceding cow. 



From tliis it would appear that subcutaneous injections of the 

 toxin of the tubercle bacillus had had some influence in increasing 

 the resistance of these two cows to feeding tuberculosis. 



E. A. deSchweinitz reported in the Medical News for December 8, 

 1894, some experiments made by him upon guinea-pigs, in which these 

 animals were inoculated with tubercle bacilli of human origin cul- 

 tivated for about twenty generations upon glycerin beef broth, and 

 were afterward inoculated with tuberculous material from a cow. 

 The guinea-pigs so treated remained free from tuberculosis, while 

 check animals inoculated with the same tuberculous material from 

 the cow died of tuberculosis within seven weeks. De Schweinitz also 

 sliowed that the twentieth generation of broth culture appeared to 

 be incapable of producing tuberculosis in a cow when she was inocu- 

 lated intravenously with a small quantity. De Schweinitz and 

 Schroeder report (U. F5. Dept. of Agr., B. A. I. Bulletin No. 13, 1896) 

 upon other inoculations similar in nature and confirmatory of the 

 above results. They show, further, that the attenuated culture they 

 were working with was not virulent for cattle when inoculated in- 

 travenously in quantities of 500 c. c. of suspension in liquid. 



The immunizing etfect upon cattle of the administration intra- 

 venously of tuberculous material or of living cultures has been 

 studied by J. McFadyean and by von Behring. 



McFadyean reported in the JouiTial of Comparative Pathology 

 and Therapeutics for June, 1901, and March, 1902, upon some experi- 

 ments regarding the immunization of cattle against tuberculosis. He 

 inoculated four cattle intravenously with emulsions of tuberculous 

 material and cfiltures from various sources. One of these cattle, 

 which had responded to the tuberculin test, and was, no doubt, tuber- 

 cular upon the beginning of the experiment, was given about 150 c. c. 

 of tuberculin in divided does before inoculation. Fifteen weeks after 

 inoculation this animal was killed and was found to contain but one 



