No. 6. DEPARTMENT OF AGRICULTURE. 147 



and the third geueratiou on blood serum after recovery supplied the 

 nialerial lor the prt,'seut inoculation. A second iutravenous inocula- 

 tion with iU c. c. ol similar suspension was made June 1, lUUl. Au- 

 gust 23, lUOl, this heifer was inoculated with 2U c. c. of a standard 

 suspension in water of a culture [H) of tubercle bacilli from human 

 sjjulum. This quantity of material was divided into four parts of 5 

 c. c. each, and these parts were injected beneath the skin, into the 

 peritoneal cavity, into the jugular vein, and into the lung, respec- 

 tively. These injections were repeated at intervals of from seven to 

 ten days until January 29, l'JU2. The (juautity of standard suspen- 

 sion was increased lU c. c. with each successive inoculation, so that 

 at the last, the eighteenth, inoculation the total quantity given was 

 160 c. c. The total quantity given in this series of inoculation was 

 1707 c. c. of standard suspension. There was a rise of temperature 

 of from two to four degrees following each inoculation after the first 

 one. The hrst inoculation caused no temperature reaction. The 

 heifer was in strong, thrifty condition at the completion of the series 

 of inoculations, and improved in condition throughout the following 

 months. It was killed August 14, 1UU2. The condition was good, 

 and there was an abundance of fat upon the carcass and about the 

 intestines. The post-mortem examination revealed the lungs to be 

 normal in color and elastic; they were free from nodules, but were 

 attached to the cliest w'alls along the lower borders by fibrous bauds. 

 A few flakes of fibrin were found upon the omentum, and these flakes 

 contained a few calcareous nodules about one-twelfth of an inch in 

 diameter. The liver was adherent to the diaphragan over an area 

 live inches in diameter. 



A yearling grade short-horn bull (2()442) after having been tested 

 with tuberculin and proven to be free from tuberculosis, was inocu- 

 lated iutraperitoneaily November lU, lUUO, with IG c. c. of a suspen- 

 sion of tubercle bacilli from a culture from human sputum that had 

 remained in a collodion capsule in the peritoneal cavity of a bull 

 for seven mouths. The third generation on blood serum after re- 

 covery furnished the material for this inoculation. March 17, 1901, 

 this bull was inoculated intravenously with 18.5 c. c. of a standard 

 suspension of a culture similar to that used in the inoculation of 

 the above heifer (2G415) on March 15 and June 1, 1901. This animal 

 was subsequently inoculated in the same manner as the heifer, re- 

 ceiving eighteen inoculations between August 23, 1901, and Janu- 

 ary 10, 1902. He received in ail 1710 c. c. of standard suspension. 

 He reacted following the inoculations very much as the heifer, al- 

 though somewhat more slowly. He remained in good condition and 

 apparent good health until he was killed, excepting for the develop- 

 ment of an abscess over the jugular vein, which was opened Novem- 

 ber 22, and afterward healed nicely. January 18, 1902, this bull was 



