148 ANNUAL REPORT OF THE Off. Doc. 



iuocLilaled iiitraperitoneally with 10 c. c. of a slaudard suspeusion 

 of lubercle bacilli from a culture (H) of boviue origiu. The viruleuce 

 of this culture for cattle had been proven by numerous iuoculatiocs, 

 among which the folio vviug may be mentioned: A cow (2G431) weigh- 

 ing 950 pomids was inoculated intravenously January 8, 1001, with 

 5 c. c. of a standard suspension from a culture of bovine tubercle ba- 

 cilli H. The cow lost weight rapidly to 750 pounds, and died March 

 4, 1001. I'ost-mortem examiuation revealed most extensive miliary 

 tuberculosis of tlie lungs. Another cow (20133), weighing 008 pounds, 

 was similarly inoculated at the same time, and died January 20 of 

 miliary tuberculosis of the lungs. This cow received two injections 

 of tuberculin of O.-l c. c. each on January 16th and 22d. Both of these 

 cows had been shown (o be free from tuberculosis by tuberculin test 

 before they were inoculated. A red heifer (45072), about eight months 

 old, was tested and did not react. It was inoculated intraperi- 

 toneally April 30, 1902, with 5 c. c. of standard suspension of bovine 

 culture H. It died June 7, 1902, and was found to contain extensive 

 lesions of tuberculosis upon the peritoneum and abdominal organs, 

 and the lungs, also, were crowded with small tubercles. The bull 

 (26442) was killed August 13, 1902. The general condition was good, 

 and there was much fat upon the carcass and about the internal 

 organs. The pleura lining the lower half of the chest was covered by 

 a sheet of partly organized fibrin from one-eighth to one-third of an 

 inch thick. The lungs themselves contained a few nodules about one- 

 half inch m diameter surrounded by thick walls and containing 

 caseous pus in which there were many tubercle bacilli. These 

 nodules did not seem to be progressive, and appeared to be abscesses 

 indicating the sites of previous inoculations. The peritoneum cov- 

 ering walls, the stomach, intestines, spleen, and liver was coated 

 with a layer of partly organized fibrin, as in the chest. The lymphatic 

 gla«ds about the rectum were enlarged and caseous. The surface 

 of the omentum was rough from the presence of a thin layer of partly 

 organized fibrin. The omentum was thickened in places, but there 

 were no distinct nodules. From the fact that the fibrinous coating 

 of the serous membranes was as pronounced in the thoracic as in the 

 abdominal cavity it is probable that the virulent culture of tubercle 

 Ijacilli iiijected into the abdomen has little to do with the production 

 of this deposit, which may readily have resulted from the discharge 

 Gf a pulmonary abscess into the pleural cavity or the discharge into 

 the peritoneal cavity of the purulent contents of one of the softened 

 lymphatic glands in the pelvis. 



It is evident that the sputum tubercle bacilli used for the inocula- 

 tion of these two animals (26415 and 26442), even in the exceedingly 

 large quantities in which they were employed, were incapable of caus- 

 ing general tubercular infection. Even the intraperitoneal inocula- 



