DEPARTMENT REPORTS. 83 



D. SANITATION. 



I. Waitr Bacteriology. 



Water from rock well and from surface well compared with sewage from river, 



1. Bacteriological Analysis. 



Agar shake (litmus lactose) to test a large quantity (100 cc.) of water 

 for acid and gas-producing germs. 



Litmus lactose agar (without salt) plates and gelatin (without salt) plates 

 inoculated with 1 cc, 0.5 cc, and 0.1 cc of water to show comparative 

 germ count and acid colonies and liquefiers. 



Broth and litmus milk tubes inoculated with 1 cc, 0.5 cc. and 0.1 cc. of 

 water to test small amounts of water for germ content and kind of 

 germs present. 



Dextrose, lactose and saccharose fermentation tubes to show gas produc- 

 tion. 



Tubes of nitrate broth and Dunham's peptone solution inoculated with 

 water germs to show the production of nitrates and indol respectively. 



Pure cultures of B. coli and B. typhosus for comparison on the above dif- 

 ferent media. 



Hanging drop of B. coli under microscope. 



2. Bacteeial Products in Water. 



Color test for nitrates, nitrites, free ammonia, and chlorine. Samples of 

 each water compared in Nessler tubes and parts per million of each 

 noted and the significance of each. 



3. Dojiestic Water Filters. 

 Unglazed porcelain. Charcoal. 



4. Charts. 



Showing contamination of water supplies. 



IT. Sewage. 



1. Bacteriological Analysis. 



As above. Drop of sewage under microscope. 



2. Bacterial Products. 



(Same as under water analysis.) 



3. Sewage Disposal. 



Aerobic — Construction of filter beds. Anaerobic — Model of septic tank. 



III. Communicable Diseases. 



1. Common Miscellaneous Soutsces of Diseases. 



Plates made from dried saliva, dirt from walks (drag a cloth one block 

 on Washington avenue to show germs gathered by long skirts), public 

 drinking cup (obtain with sterile moist swab and smear over sterile 

 agar), public telephone — receiver and transmitter — (use sterile moist 

 swab), towel used by the public (soak part in sterile water), door knobs 

 and jambs (use sterile moist swab), infected cloth, before and after 

 boiling, before and after ironing, hands (washed in sterile water and 

 with some disinfectant), public comb, razor, food exposed to dust of 

 street, pet animals, library books, money, etc. 



2. Bacteria in Air. 



Plates containing sterile agar exposed one minute to — outside air (campus 

 and city of Lansing), air in lower corridor Women's Bldg. while girls 

 are going to classes, air of room (before, during and after sweeping), 

 air of room before, during and after cleaning with vacuum cleaner, air 

 of room (dusting with dry and damp cloth). 



List of diseases transmitted by air. 



3. Microscopic Demonstration of Sources. 



Stained specimens of saliva — tuberculous sputum. Tartar from the teeth. 

 Public drinking cup. Public telephone. Pus direct from suppurating 

 wounds. 



4. Insect Carriers of Disease. 



House fly. Musca domestica.. Diseases transmitted by house fly — typhoid 

 fever, dysentery and all other intestinal diseases, tuberculosis. Chart 

 to show how flies carry disease by means of their feet, also by means 

 of their excreta. The house fly does not bite. Fly posters. Fly tracks 

 on agar plate. Breeding place and rapidity of breeding on placard. 



