DEPARTMENT REPORTS. 85 



IV. Individual Species. 



There are certain bacterial species of prominent importance in the soil. 



1. PSEUDOMONAS RADICICOLA. 



The organisms of commercial cultures for inoculating legumes. Known 

 also as legume or nodule-forming bacterium. Growth of nodule on root 

 showing its prevalence on all legumes. Demonstration of failure of 

 cross inoculation. Characteristics of the species shown by stained slides, 

 cultures on special media. Composition of media. Commercial produc- 

 tion of cultures showing method of obtaining bacteria from nodule, test- 

 ing of these bacteria on plants grown from sterile seeds and on sterile 

 agar or quartz sand. Methods of seed sterilization. Commercial cul- 

 tures of organisms for alfalfa, alsike clover, red clover, white clover, 

 vetch, beans, soy beans, peas, cowpeas, sweet peas. Method of shipping 

 the cultures. Charts showing the distribution of the cultures through- 

 out the state. 



2. AZOTOBACTER. 



An organism which assimilates nitrogen from the atmosphere. Prevalence, 

 results from inoculation. Cultures, plates, and slides to show its charac- 

 teristics. 



3. Other Species. 



Such as ammoniflers (decomposing organic matter to ammonia), nitrifiers 

 (forming nitric acid), denitrifiers (breaking down nitric acid), etc. 



F. ANIMAL PATHOLOGIC BACTERIOLOGY. 



I. Disease Peoducing Organisms. 



1. Cut.tuees of Disease-producing Organisms. 



Specific: 1. Bad. tuberculosis. 2. Bad. anthrads. 3. Bad. abortus 

 4. Bad. mallei. 5. B. cTiolerae suis. 6. Bad. diphtheriae. 7. B. typhosus, 

 etc. 



Non-specific: Pus-producing organisms. 



2. Microscopic Demonstration. 



Bad. tuberculosis, human, bovine, avian. B. typhosus, Bact. diphtheriae, 

 pyogenic bacteria. 



3. PiiOTo:MiCEOGRAriis OF Disease-producing Bacteria. 



Bact. tuberculosis in sputum. Bact. diphtheriae. Negri bodies, (found in 

 hydrophobia or rabies). Gonococcus. B. typhosus. Bact. abortus (Bang) 

 from cotyledon. 



Photographs showing — Turkey sick of entero-hepatitis or blackhead. Hen 

 with infected feather follicles. Macroscopic agglutination of B. cholerae 

 suis. Subdural inoculation of rabbit, etherized. Intraperitoneal inocu- 

 lation of rabbit. Subcutaneous inoculation of rabbit. Inoculated rabbit 

 in cage. Weighing guinea pig before treatment. 



II. Diseases. 



1. Tuberculosis. 



Bovine — tubercles in lung, lymph glands, mammary glands, liver, spleen, 

 heart, peritoneum. Porcine- — tubercles in skin, bone, joints, mammary 

 glands, testicle, adrenal, submaxillary lymph gland, lungs, liver, heart. 

 Avian — tubercles in lung, liver, spleen, intestine. 



Photographs of healthy and tuberculous cows, the latter both apparently 

 healthy and visibly diseased. Outfit for collecting human tuberculous 

 sputum and method for demonstrating presence of germs. 



2. Hog Cholera. 



Diseased organs in hog cholera. 



3. Other Animal Diseases. 



Actinomycosis (lumpy jaw) in head, liver, diaphragm of cattle. Infec- 

 tious abortion — diseased cotyledons. Entero-hepatitis (black head) in 

 turkeys. Tumors and other pathological conditions. Animal parasites 

 and their effect on animals. 



4. Maps. 



showing distribution of hog cholera, tuberculin testers and contagious 

 abortion of cattle In Michigan. 



