168 STATE BOARD OF AGRICUI-TURE. 



had been reserved as the most probably affected. One of. them died 

 shortly after receipt and showed, on autopsy, tubercular liver, spleen 

 and intestines. We decided to try the tuberculin test on the remainder. 

 Six healthy white leghorn hens were introduced from the college flock 

 as checks. The Giovemment (B. A. I.) tuberculin was used. Tables 



1 and II show the results of the tests on both the normal birds from 

 the college flock and the normal and tubercular birds from Chilson, 

 Initial temperatures were taken on Feb. 22, 23, 24 and at 8 a. m., Feb. 

 25th. Three normal college birds were then injected with, 0.1 cc. and 

 0.5 cc. of tuberculin diluted ten times with 0.5 per cent carbolic acid 

 respectively, and one with 2 cc. of undiluted tuberculin. Also six of 

 the Chilson chickens were injected, one with 0.1 cc, one with 0.2 cc, 

 and one with 0.5 cc of diluted tuberculin, and one with 1 cc, one with 



2 cc, and one with 5 cc. of undiluted tuberculin. The temperature 

 of these birds was then taken throughout that day and the following. 

 The remaining birds were injected at 9 p. m. on Feb. 25th with 1 cc. 

 each of undiluted tuberculin. The injection wiis made into the pectoral 

 muscles in all cases. 



The results confirm the work of Ward in this connection, and show 

 the inajiplicability of the tuberculin test with chickens. Ward ob- 

 tained similar results with avian tuberculin, i. e., tuberculin prepared 

 from avian tubercle bacilli. 



There is a greater variation in the temperatures of the normal birds 

 than in the tubercular. Absolutely nothing can be deduced from these 

 records that will serve as a clue to further work along this immediate 

 line of investigation. The problem must be approached from a differ- 

 ent angle. 



Autopsy records showed the first eight chickens in Table II to be 

 free from gross lesions of tuberculosis. 



No. 19 showed emaciation — liver, intestine, spleen affected. 



No. 309 showed emaciation — liver, intestine, spleen affected. 



No. 258 showed emaciation — liver, intestine, spleen affected. 



No. 903 showed intestine slightly affected. 



No. 560 showed liver, intestine, meseutei'y, ovary affected. 



No. 895 showed intestine and liver affected. 



No. 643 showed emaciation; liver, intestine and spleen affected. 



No. 74 showed emaciation; intestines affected. 



No. 457 showed emaciation; liver affected. 



No. 30 showed emaciation ; liver affected. 



Of these cases : 



Four or 40 per cent, showed liver, intestine and spleen affected. 



Two or 20 per cent, showed intestine and liver affected. 



Two or 20 per cent, showed liver only affected. 



Two or 20 per cent, showed intestine only affected." — [Ward Giltner.] 



A Disease of Sheep in Clinton County. — "Dr. Gohn has reported losses 

 of ewes advanced in pregnancy for a number of years. Almost with- 

 out exception, the ewes have carried twin lambs. The mortality in 

 affected cases has been ])ractically 100 per cent, but only a small per- 

 centage of a flock as a rule has suffered. The sick animals lose their 

 appetite, persist in n recumbent attitude with the head drawn to one 

 side, as in milk fever in cows, and the neck muscles are tensely con- 



