EXPERIMENT STATION BULLETINS. 411 



REACTIONS WITH NORMAL SERA. 



We have already recorded the reactiions with the blood of 9 normal 

 pigs. Of these, 3 gave a reaction at 1-125, the remaining 6 gave only a 

 slight reaction at 1-50. In Table I are recorded the reactions with the 

 blood of 32 normal* pigs. Of the 32 samples of normal blood tested, 



6 or 18.75 per cent gave no reaction at a dilution of 1 — 25. 



6 or 18.75 per cent gave a reaction at a dilution of 1 — 25. 



10 or 31.25 per cent gave no reaction at a dilution of 1 — 50. 



3 or 9.375 per cent gave a reaction at a dilution of 1 — 50. 



5 or 15.625 per cent gave a reaction at a dilution of 1 — 125. 



2 or 6.25 per cent gave a reaction at a dilution of 1 — 250. 



In the second column of Table I are given the weights of the pigs at 

 the time the sample of blood was drawn. It was fiound that, in the 

 former records, of the three normal pigs whose blood gave a reaction 

 at 1-125 that the average weight was 66 lbs., and of the six normal pigs 

 whose blood gave a reaction at 1-50 the average weight was 23 lbs. We 

 find from a study of Table I that of the 



6 pigs giving no reaction at 1 — 25 the average weight is' 39 lbs. 

 6 pigs giving a reaction at 1 — 25 the average weight is 47 lbs. 



10 pigs giving no reaction at 1 — 50 the average weight is 31 lbs. 



3 pigs giving a reaction at 1 — 50 the average weight is 37 lbs. 

 5 pigs giving a reaction at 1 — 125 the average weight is 63 lbs. 

 2 pigs giving a reaction at 1 — 250 the average weight is 52 lbs. 



We see in these results a tendency for the agglutinative power of the 

 seinim to increase with the size or more probably the age of the pig. 

 This point cannot be considered determined by the few tests recorded 

 here. The results, however, are suggestive. 



REACTIONS W^TII THE BLOOD OP PIGS TREATED WITH VIRUS. 



Table II shows the agglutination reactions with the blood of 50 

 pigs inoculated with virus of hog cholera, i. e., with blood drawn 

 from a pig showing symptoms and lesions of hog cholera. There is 

 also shown a considerable amount of other data included with the 

 hope that the interpretation of the agglutination reactions may be 

 facilitated. 



*The word "normal" is used in tlie sense that tlie pig has neitlier l)een exposed to hof; cholera nor 

 subjected to anj' treatment. 



