EXPERIMENT STATION BULLETINS. 



429 



Date of test. 



11-22-09 



12- 3-09 



12- 9-09 



12-13-09 



12-20-09 



12-27-09 



1- 3 10 



1 10 10 



1-17 10 



1-24-10 



1-3(M0 



Amount of L-, Maximum, 

 virus injected dJ"tion at which 

 ■ ■ agglutination 



occurred. 



to date. 



50 cc. 



250 CO. 



725 cc. 

 1175 cc. 

 1175 cc. 

 1750 cc. 

 1750 ce. 

 1750 cc. 

 2050 cc. 

 2050 cc. 

 2050 cc. 



1^00 



1^00 



1-1000 



1-4000 



1-4000 



1-4000 



1-4000 



1-2000 



1-50,000 



1-25,000 



1-12,500 



Bleeding. 



5 ec. from tail. 

 5 cc. from tail. 

 5 cc. from tail. 

 5 cc. from tail. 

 First tail. 

 Second tail. 

 Third tail. 

 Fourth tail. 

 Fifth tail. 

 Killed. 

 Mixed 1—6. 



A total injection of 50 cc. caused a reaction at 1-400; an increase of 200 

 cc. produce.s no change. A total of 725 cc. gives a reaction at 1-1,000, 

 while a total of 1175 cc. results in a reaction at 1-4000 which is main- 

 tained until the first bleeding. An increase of 575 cc. produces no 

 change in the agglutinative reaction for the second and third bleedings 

 and results in a fall to 1-2000 at the fourth bleeding. Another injection 

 of 300 cc. brings the reaction up to 1-50,000 but at the last bleeding it 

 falls to 1-25,000. The reaction produced by a mixture of the different 

 bleedings is about an average of the reactions for all. 



The increase in virus injected seems to result in an increase (not 

 necessarily pari passu) in the agglutinin content of the serum. The 

 withdrav\'al of a considerable amount of blood tends to produce a de- 

 crease in the agglutinin content which may or may not be offset by 

 vinis injections. It is true that the response is very irregular as can be 

 seen by studying the tables. 



It is not maintained that it is the blood injected or the ultravisible 

 virus that stimulates the production of agglutinins but the B. cholerae 

 siiis in the blood. In other words we assume that it is the number of B. 

 cholerae siiis in a quantity of virus and not the volume that aft'ects the 

 agglutinin production in the treated pig. 



Unfortunately we were not in a position to make bacterial counts 

 of any great number of samples of virus. We have not found above 

 10,000 bacteria per cc. in fresh virus. If all these organisms were 

 B. cJiolerae suis the total number in 3,000 cc. of virus would be 30,000,- 

 000. We have estimated (by a number of platings) that 1 cc. of a 24 

 hour bouillon culture of B. cholerae suis contains about 500,000,000 

 bacteria. We have not used so much as 3,000 cc. of \irus on any of 

 the 19 cases studied, therefore we have probably not injected more 

 B. cholerae suis than would be found in .10 cc. of a 24 hour bouillon 

 culture. 



In one case we found that the injection of 10 cc. of a 24 hour bouillon 

 culture of B. cholerae suis ^'virus 136" resulted in a serum giving a 



