460 



STATE BOARD OF AGRICULTURE. 



For further data see also tables XXI, XXII, XXIII, XXIV. 



The difference in the fermenting capacity of young and old cultures 

 will be evident also in cultures of the same a.ge, but with different 

 amounts of inoculum. A good many experiments in this paper have 

 been carried on in duplicates with different amounts of inoculum, and 

 the result is that in all cases the lowest inoculum showed the highest 

 fennenting capacity, being actually the younger culture. This is especi- 

 ally plainly seen in Table V. 



Effect of Neutralization. — To obtain old cells unchecked by their own 

 products, full-grown milk cultures were neutralized with sterile nor- 

 mal alkali to the normal acidity of milk. A milk culture of Strain IT, 

 3G hours old, 80° acid, was neutralized to 11°. The bacteria were count- 

 ed immediately before and one-half hour after neutralization, and then 

 after 4 and 8 hours. The experiment was carried on in duplicate. 



TABLE VII. — Growth and Acid Production of Strain II. 



