486 



STATE BOARD OP AGRICULTURE. 



TABLE U. -Summary of Tests VII-XII Inclusive. 

 DIFFERENTIAL COUNTS ON OX-BILE AND CALCIUM-CARBONATE AGAR RESPECTIVELY. 



Xiimlicr of test. 



Age. of fonil)ined Piiltiire. 



Acidity of 



culture at 



time of 



estimate. 



Number of liactcria in eacli 



cubic centimeter of milk 



at the times specified. 



B. typhosus. 



Bad. ladis 

 aciili (kib.) 



VII. 



VIII. 



IX. 



XI. 



XII. 



hours . . . 



22 hours . . 

 7(i.5 hours 



hours . . 



23 liours . . 

 4().5 hours 



liours. . 

 21 hours. . 

 47 hours . . 



hours . . 

 23 hours . . 

 40 hours. . 



hours . . 

 39 hours . . 



hours . . 

 26 hours . . 



15' 

 73° 



82° 



15° 



02° 

 08° 



15° 



or 



70° 



15° 



08° 

 80° 



1.5° 

 71° 



15° 

 04° 



2, 034,, 575 



119,000,000 



11,000,000 



1,898,000 

 24,467,000 



None. 



5,025,000 

 107,000,000 



None. 



1,775,000 



35,200,000 



050,000 



2,385,000 

 0,500,000 



2,215,000 

 5,400,000 



20S,293 

 290,000,000 

 Counts lost. 



1,. 580. 000 

 .3,52,000,000 

 424,000,000 



3,550,000 

 229,800.000 

 300,000,000 



1,075,000 



334,100,000 



97,000,000 



1,420,2,50 

 118,000,000 



1,077,500 

 115,000,000 



From tlie above table, it will be noted that the lactic acid ])roduced 

 by Jiact. hicti.s acull (lab.) becomes inhibitive 1o />* ti/plioxiix from 24-48 

 hours after inoculation when the acidity at that time has reached (;8°- 

 80° or over. 



It will be noted also, in this table, that a higher degree of acidity and 

 a longer time exists before the inhibitive effect takes place than in the 

 previous table. This is easily accounted for by the fact that more ac- 

 curate results were obtained by use of the cheek plates. 



Tests I-XIT were carried on with Bact. lactis acidi (lab.). As we were 

 desirous of obtaining other types of lactic organisms for c(nn])arison with 

 the one used in the above experiments, through the courtesy of Dr. 

 Heinemann and Dr. Hefferan of the University of Chicago, we were fur- 

 nislicd with several cultures of lactics. 



The following are the names of the lactic organisms used in the suc- 

 ceeding experiments : 



lidct. aoidi hicticd, Hueppe. 



Jiact. acidi kictici, Novy. 



liact. (icidi lactici No. 1. 



liact. acidi laciici No. 2. 



^trept. lacticus. 



At this time, several different lactic cultures were received from Dr. 

 Harding of the New York PTxperiment Station at Geneva. Only one was 

 used in the following exi)eriments, Bact. luetic acidi, Harding. 



As the supj)Iy of ox-bile agar was exhausted at this time, the third 

 lot of experiments was carried on with sheep-bile agar. 



It will be noted also that the numbei- of typhoid and of lactic micro 

 ortxanisms on each medium is recordiMl. 



