EXPERIMENT STATION BULLETINS. 



497 



As seen by Table XVI, the same amount of acid as produced by differ- 

 ent lactic organisms has a different efifect on the longevity of the typhoid 

 bacillus (see especially 45° and 48° acid). Whether this is due to the 

 natural variation which takes place in different cultures of the same 

 organism (see 46° and 60° acid) or whether it is due to the difference 

 in the by-products other than lactic acid, of the different lactic organisms, 

 is still a matter of question. 



However, a certain uniformitv in the inhibitive effect of the fermenta- 

 tion lactic acid is shown by Table XVI. From 60°-68° acid, the prod- 

 ucts of four different lactic organisms kill the typhoid bacteria within 

 from 19 to 30 hours, i. e., within 24 hours on the average. 



TABLE XVII. 



Lactic organism. 



Bad. acidi ladici ''Lactone" 

 Bad. ladis acidi (lab.) 



Bad. ladis acidi (from whey) . 



Bad. ladis acidi (from cheese) . 



Bad. ladis acidi, Harding. 



Bad. addi ladici. No. 2 . 



Bad. ladis addi (from starter) . 



Strept. ladieus . 



Bad. ladis addi (stock) . 



No. 



of 



test. 



Ill 

 II 



II 



III 



I 



II 



ni 

 I 



I 

 II 



III 



V 

 IV 



IV 

 V 



I 



III 

 II 



I 

 II 



V 



III 



IV 



V 

 VI 

 VII 



III 



IV 



II 



V 

 VI 



I 

 in 



Acid- 

 ity of 

 lactic 

 cul- 

 ture. 



40° 



32° 

 42° 



40° 

 47° 

 48° 



41° 



48° 

 50° 



45° 

 54° 

 60° 

 60° 

 62° 



Number of typhoid bacilli per cc. of filtrate. 



Intro- 

 duced. 



178,650 



82,830 

 59,550 



139,500 



209,250 



57,000 



156,000 



136,700 



57,340 



106,780 



88,920 



79,290 



9,960 



58,950 



2nd plating. 



540,000 



183,000,000 

 None 



38,700,000 



Uncountable. . . 

 11,610 



1,310,000 



None 



9 



23? 



None 



None 



2 

 None 



186 



None 



12,170 



175 



50 



4 



None 



None 



None 



None 



None 



None 



None 



None 



None 



None 



None 



None 



None 



None 



3rd plating. 



Not plated.. 



Not plated.. 

 None 



Not plated.. 



Not plated.. 



11,780 



Not plated.. 



None 



Not plated.. 



None 



None 



None 



None 



None 



None 



Not plated.. 



None 



None 



None 



Not plated.. 



None 



Not plated.. 

 Not plated.. 

 Not plated.. 



None 



Not plated.. 

 Not plated.. 

 Not plated.. 

 Not plated.. 



None 



None 



None 



Not plated.. 

 Not plated., 



Remarks. 



Increased in 29 hours. 



Increased within 24 hours. 

 Killed within 20 hours. 



Increased within 19 hours. 



Increased within 22.5 hrs. 



See note *under Table XVI 



Increased within 24 hours. 

 Killed within 25 hours. 

 Decreased within 26 hours. 



Killed within 53 hours. 

 KUled within 30.5 hours. 

 Killed within 29.5 hours. 

 Killed within 46 5 hours. 

 Killed within 23.5 hours. 



Killed within 46.5 hours. 

 Killed within 24 hours. 

 Killed within 49 hoiu-a. 

 Killed within 44.5 hours. 

 Decreased within 19 hours. 



Decreased within 22.5 hrs. 

 Killed within 29.5 hours. 

 Killed within 24 hours. 

 Killed within 22.5 hours. 

 Killed within 25.5 hours. 



Killed within 22.5 hours. 

 Killed within 26.5 hours. 

 Killed within 26.5 hours. 

 Killed within 19 hours. 

 Killed within 22.5 hours. 



Killed within 25.5 hours. 

 Killed within 22.5 hours. 

 Killed within 22.5 hours. 

 Killed within 25 hours. 

 Killed within 25.5 hours. 



63 



