128 ANNUAL REPORT OF THE Off. Doc. 



and potatoes, and showed in five days the characteristic growth of a 

 Sporothrix. In two other instances the same micro-organism was 

 isolated from the same liorse. After being tested on many different 

 culture media and thoioughly identified, animal inoculations were 

 made and confirmed the views expressed by Page, Frothingham and 

 Paige. In two instances the agglutination according to Widal was 

 positive 1:800. Another sample of pus and serum from a horse 

 quarantined in Pennsylvania gave the same result. The complement 

 fixation was negative with glanders antigen and positive with sporo- 

 thrix extract. The histological structire was identical with those 

 described for this disease in human beings, and there is no doubt but 

 that the disease which resembled ''Epizootic Lymphangitis" is Sporo- 

 thrilcosis. The investigation in connection with true Epizootic 

 Lymphangitis was contained. Cultures of the real Saccharomyces 

 farcinomosus were promised to the laboratory from Africa and Italy. 

 They will be the basis for a complete comparative study. It may 

 be mentioned here that Sporothrix cultures were undoubtedly ob- 

 tained from many other cases at the laboratory previous to this 

 investigation, because several culture tubes found in the laboratory 

 proved on replantation on the necessary media to be identical with 

 those of Sporothrix. The facts will be published in two or three 

 months in connection with work done in other laboratories on a 

 blastomycotic skin disease of dogs. 



3. TUBERCULOSIS 



The research work outlined and started by Dr. Leonard 

 Pearson and continued by Dr. Gilliland was completed; the 

 animals were tested, destroyed and autopsied. The results, at 

 present in a stage of compilation, will be included in the report on 

 ''The Tuberculosis Vaccination according to the method of Dr. Pear- 

 son." 



Many interesting features were observed during these autopsies. 

 Animal inoculations were made and the tubercle bacillus isolated to 

 determine its biological characteristics and its virulency to cattle. 

 To verify the views advanced by Prof. Eber many different tests must 

 necessarily be carried out before an opinion can be expressed. This 

 work proved that we are still in an experimental stage in the ques- 

 tion of immunizing cattle against tuberculosis. This and other rea- 

 sons collected from various investigators caused us to abandon the 

 vaccination of cattle against tuberculosis for the present. 



Ophthalmic Test. Recently German publications have called at- 

 tention to the good results obtained with several newer methods 

 of tuberculin testing. Such tests were applied under the different 

 conditions in Pennsylvania to determine whether or not they are of 

 value to the general routine of tuberculin testing. The ophthalmic 

 test has been applied on many animals and the results were con-- 

 trolled by the subcutaneous test and autopsy. The value of the ocular 

 test was confirmed, and we hope to be able soon to apply it more uni- 

 versally in coniunction with the subcutaneous tuberculin injection. 

 The special tuberculin required for the ocular test has been prepared 

 and can be supplied at any time when wanted. The results of some 

 work done on this subject have been published in the proceedings of 

 the United States Livestock Sanitary Associations under the title of 

 "Newer Methods of Tuberculin Testing" in December, 1911. 



