No. 6. DEPARTMENT OF AGRICULTURE. 138 



GLANDERS 



One hundred and tAventy-four specimens were received for exami- 

 nation, one hundred and three being samples of serum and twenty- 

 one of pus. The examination of these specimens was determined 

 as follows: 



Diagnosis of serum: 



1. Complement deviation test. 



2. Agglutination test. 



Diagnosis of pus: 



1. Microscopical examination. 



2. Strauss method. 



These tests were carefully carried out in each instance, and checked 

 with the record of the mallein test, if such had been made. When- 

 ever circumstances would permit an autopsy was made and record 

 kept of each examination. 



The complement deviation test, heretofore not carried out at the 

 laboratory, has proven invaluable in the diagnosis of this disease. 

 In each instance, however, an agglutination test was also made, and 

 results checked up. As before proven, it was found that the aggluti- 

 nation test alone is of very little value. The Strauss reaction wais 

 made from samples of pus, and when necessary the bacillus maillei 

 was isolated to confirm the diagnosis. 



During the year a diagnosis of fifteen cases of glanders was made. 

 Several fine pathological specimens from glandered horses were 

 brought to the laboratory by veterinarians and added to the exhibit 

 in the museum. They consisted of sections of lungs, spleen and nasal 

 septum. 



HOG CHOLERA 



A total of sixty specimens representing twentj^-five different hogs, 

 and including three whole carcasses were received and examined. 

 A positive diagnosis was made in twenty-two cases, two were reported 

 as negative. 



TUBERCULOSIS 



Thirty-four specimens exclusive of milk samples were received 

 and examined. These specimens including different tissues, pus and 

 feces and the diagnosis depended upon were: 



1. (a) The demonstration of tubercle bacilli in the smears. 



(b) Examination of smear preparations after the antiformin 

 method. 



2. The microscopical examination of tissue. 



3. The animal inoculation test. 



Twenty-three specimens were diagnosed positive and fourteen nega- 

 tive. Several gross specimens w^ere received from the School of Veter- 

 inary Medicine and the different abattoirs. They were properly 

 mounted, and added to the museum collection. 



