No. 6. DEPARTMENT OF AGRICULTURE. 135 



Tuherculin: One hundred and ninety-eight thousand seven hun- 

 dred and twenty cubic centimeters of tuberculin solution were pre- 

 pared and distributed. Over six hundred cubic centimeters of con- 

 centrated tuberculin were used in the preparation of dry precipitated 

 tuberculin for the ophthalmic test. 



Mallein: Two thousand seven hundred and sixty-eight c. c. of 

 mallein solution were prepared during the year. 



Anthrax Vaccine: One thousand six hundred and seventy-one 

 doses of anthrax vaccine Nos. 1 and 2 for cattle, and fifty-one doses 

 of anthrax vaccine Nos. 1 and 2 for horses were prepared and dis- 

 tributed. The reports following these vaccinations were very favor- 

 able. The cultures of the different anthrax strains, from which the 

 vaccines are made, are transplanted upon new culture media regu- 

 larly. New standardized vaccines are freshly prepared early each 

 Spring for the annual vaccinations. 



Hog Cholera Serum: Two hundred and seventeen thousand and 

 eighty-five c. c. were manufactured and distributed during the year. 



Computing the Hog Cholera serum at 5 cents per c. c, the tuber- 

 culin and mallein at 15 cents per dose, and the anthrax vaccine at 

 10 cents per dose, the saving to the Commonwealth for these pro- 

 ducts alone is |19, 138.00. It is not possible to estimate in dollars 

 and cents the livestock saved by their application. 



3fi1Jc Hygiene: In addition to the regular work for the State an 

 experiment was conducted for the United States Department of Agri- 

 culture, entitled "Comparative Studies of Biochemic Eeactions of 

 Milk to the Bacterial Count and Their Practical Application." One 

 hundred samples were collected from various stores throughout the 

 city of Philadelphia to compare the biochemic reactions with the 

 bacterial count. Several experiments were conducted to observe the 

 conditions which influences the several biochemic reactions used. 



Weekly bacteriological examinations were made of samples of 

 certified milk and cream from different dealers around Philadelphia. 

 All samples of milk that contained more than ten thousand bacteria 

 per cubic centimeter, and all samples of cream that contained more 

 than twenty-five thousand bacteria per cubic centimeter were reported 

 to the Secretary of the Philadelphia Pediatric Society. The maxi- 

 mum bacterial count of this Society for milk is ten thousand per cubic 

 centimeter, and for cream twenty-five thousand per cubic centimeter. 

 When the count exceeds these figures the Society may withdraw its 

 certification. 



A microscopic examination is also made of different types of bac- 

 teria to determine whether any are pathogenic. If the colonies are 

 found to be of a pathogenic type, the Secretary is notified, and an 

 inspection made of the herd. 



One hundred and fifty-four samples of milk were sent to the labora- 

 tory by veterinarians and farmers and examined during the year. 

 The examinations consisted of a determination of the number and 

 kind of bacteria present, the specific gravity, per cent, fat, per cent, 

 of acid, per cent, of total solids, preservatives, etc., or for a diagnosis 

 of pathological conditions. 



Respectfully submitted, 



K. F. MEYER, 



Director. 

 To C. J. Marshall, State Veterinarian. 



