642 STATE BOARD OF AGRICULTURE. 



G. Temperature Relations: 



(a) Growth at room temperature. 



(b) Favored by 37 degrees C. 



(c) Not killed by heating at 120 degrees C. for ten 



minutes. 



(d) Killed by heating at 120 degrees C. for twenty min- 



utes. 



Bacillus B. 

 (Conforms to description of B. siihtilis as given by Ford.) 

 Found in cans A I 1, A I 2, A I 3, A I 4, A I 5, A V 11, A V 12, A V 13. 



I. Morphology : 



1. A^egetative Cells; 



(a) Rod. 



(b) Size — 2.5-4.5 x 0.5-0.75 microns. 



2. Endospores : 



(a) Position — Central or nearly so. 



3. Motility : 



(a) Actively motile. 



4. Staining : 



(a) Gram positive. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics : 



1. Gelatin Stab: 



-(a) Top growth, 

 (b) Liquefaction — Nearly crateriform. 



2. Nutrient Broth : 



(a) Heavy top growth not easily broken. 



(b) Broth nearly clear, yellowed. 



3. Litmus milk: 



(a) Peptonization. 



(b) Alkaline. 



4. Glycerin Potato: 



(a) Very abundant growth. 



(b) Dull, rugose. 



(c) Cream colored. 



5. Gelatin Agar Colonies : 



(a) Growth — Rapid. 



(b) Granular. 



(c) Rhlzoid. 



(d) White. 



