EXPERIMENT STATION BULLETINS. 643 



III. Physical and Biochemical Features: 



1. Fermentation tubes, neutral red broth plus 



(a) Dextrose: 



Gas — None. 



E ea c tion — A cid . 



Heavy top growth. 



(b) Lactose: 



Gas — None. 

 Reaction — Alkaline. 

 Heavy top growth. 



(c) Saccharose: 



Gas — None. 

 Reaction — Acid. 

 Top growth. 



2. Peas in distilled water: 



(a) Aerobic culture: 



Gas production. 



Cream colored, wrinkled growth, darkens. 



Slime i)roduction. 



(b) Anaerobic culture: 



Gas production. 

 Slime. 

 Yellowish liquid. 



3. Peptone Solution : 



(a) Indol formation. 



4. Nitrate Peptone Solution: 



(a) Nitrate reduction. 



(b) Ammonia production. 



5. Starch Peptone Solution : 



(a) Complete reduction of starch. 



(b) Large quantity of sugar. ■ 



(c) Solution, slightly alkaline. 



6. Temperature Relations : 



(a) Grows readily at room temperature. 



(b) Favored by 37 degrees C. 



(c) Not killed by heating at 120 degrees C. for ten 



minutes. 



(d) Killed by heating at 120 degrees C. for twenty min- 



utes. 



Bacillus C. 

 (Resembles B. hotuUnus morphologically and culturally.) 

 Found in can A I 2. 

 I. Morphology: 



1. Vegetative Cells: 



(a) Large rod with rounded ends. 



(b) Size — 4-G x 0.75 microns. 



