644 STATE BOARD OF AGRICULTURE. 



2. Endospores : 



(a) Position — Polar or sub-terminal making the rod 

 club-shaped. 



3. Motility : 



(a) Actively motile. 



4. Staining: 



(a) Gram positive. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics : 



1. Nutrient Broth under oil : 



(a) Broth cloudy. 



(b) Odor of butyric acid. 



2. Gelatin Agar Colonies: 



(a) Anaerobic. 



(b) Furry. 



(c) Whitish, becomes somewhat tan. 



3. Dextrose Gelatin Agar Shake: 



(a) Gas at 25 degrees C. 



(b) No gas at 37 degrees C. 



III. Physical Characteristics: 



1. Temperature Relations: 



(a) Growth best at 25 degrees C. 



(b) Slow growth at 37 degrees C. 



(c) Not killed by heating at 120 degrees C. for ten 



minutes. 



(d) Killed by heating at 120 degrees C. for twenty 



minutes. 



Bacillus D. 

 Found in can A I 2. 



I. Morphology : 



1. Vegetative Cells: 



(a) Slender rods. 



(b) Size — 1.5-3 x .5 microns. 



2. Endospores : 



(a) Position — Central. 



(b) Slight enlargemeut of the rod on sporulatiou. 



(c) Spores — Oval. 



3. Motility: 



(a) Actively motile. 



4. Stain: 



(a) Gram negative. 



(b) Stains with ordinary dyes. 



