EXPERIMENT STATION BULLETINS. (j45 



II. Cultural Characteristics : 



1. Gelatin Stab: 



(a) Liquefaction — Infundibuliform. 



2. Nutrient Broth : 



(a) No top growth, 



(b) Cloudy. 



(c) Sediment. 



3. Litmus milk. 



(a) Peptonization. 



(b) Alkaline. 



4. Glj'cerin Potato: 



(a) Flat, smooth, glistening growth. 



(b) Color, creamy tan. 



5. Gelatin Agar Colonies : 



(a) Flat. 



(b) Spreading. 



(c) White, turning tan in forty-eight hours. 



Ill, Physical and Biochemical Features: 



1. Fermentation tubes, neutral red broth plus 



(a) Dextrose: 



Gas — None. 

 .Keaction — Slightly acid. 



(b) Lactose: 



Gas — None. 

 Reaction — Alkaline, 



(c) Saccharose: 



Gas — None. 

 Reaction — Acid, 



2. Peas in distilled water: 



(a) Aerobic culture: 



Gas, 



Creamy top growth, 



(b) Anaerobic culture: 



Gas, 



8. Peptone Solution : 



(a) No indol production. 



4. Nitrate Peptone Solution: 



(a) Nitrate reduction, 



(b) Ammonia production. 



5. Starch Peptone Solution: 



(a) None or very slight reduction of starch. 



(b) No production of sugar, 



(c) Slight acid. 



