646 STATE BOARD OF AGRICULTURE. 



6. Temperature Relations: 



(a) Growth both at 25 degrees and 37 degrees C. 



(b) Not killed by heating at 120 degrees C. for ten 



minutes. 



(c) Killed by heating at 120 degrees C. for twenty 



minutes. 



(d) AVithstands 3I/2 hours heating in flowing steam. 



Bacillus E. 

 (Resembles B. ramosus.) 

 Found in cans A V 10, and A V 12. 



I. Morphology : 



1. Vegetative Cells: 



(a) Thick rods. 



(b) Size — 2-3.5 x 0.75-1 microns. 



2. Endospores : 



(a) Position — Central. 



3. Motility : 



(a) Motile, not active. 



4. Staining : 



(a) Gram positive. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics: 



1. Gelatin Stab: 



(a) Liquefaction — Crateriform to stratiform. 



(b) Whitish sediment which turns pinkish. 



2. Nutrient Broth : 



(a) Top growth flaky which settles on shaking. 



3. Litmus milk : 



(a) Peptonization. 



(b) Alkaline. 



4. Glycerin Potato : 



(a) Growth — Abundant, raised, contoured. 



(b) Moist, creamy to pink, becoming purplish. 



5. Gelatin Agar Colony: 



(a) Flat. 



(b) Sometimes somewhat spreading. 



(c) White, translucent. 



III. Physical and Biochemical Features: 



1. Fermentation tubes, neutral red broth plus 

 (a) Dextrose: 



Gas — None. 

 Reaction — Acid. 



