EXPERIMENT STATION BULLETINS. ' 647 



(b) Lactose: 



Gas — None. 



Reaction — Slightly alkaline. 



(c) Saccharose: 



Gas — None. 

 Reaction — Acid. 



2. Peas in distilled water : 



(a) Aerobic culture: 



Gas. 



Somewhat rose colored top growth. 



(b) Anaerobic culture: 



Gas. 



3. Peptone Solution: 



(a) Indol formation. 



4. Nitrate Peptone Solution : 



(a) Nitrate reduction. 



(b) Ammonia formation. 



5. Starch Peptone Solution : 



(a) Slight reduction of starch. 



(b) Slight test for sugar. 



G. Temperature Relations: 



(a) Grows readily at either 25 degrees or 37 degrees C. 



(b) Not killed by heating at 110 degrees 0. for ten 



minutes. 



(c) Killed by heating at 110 degrees C. for thirty 



minutes. 



Bacillus H. 

 Found in cans A I 3, and A I 1. 



J. Morphology : 



1. Vegetative Cells: 



(a) Rods usually in long chains. 



(b) Size — 2.5-3 x 0.5 microns. 



2. Motility: 



(a) Slowly motile. 



3. Endospores : 



(a) Central. 



4. Staining : 



(a) Gram positive. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics : 



1. Gelatin Stab: 



(a) Liquefacti©n — Crateriform. 



