648 STATE BOARD OF AGRICULTURE. 



2. Nutrient Broth: 



(a) No top growth. 



(b) Medium cloudy. 



(c) Fine precipitate. 



3. Litmus milk: 



(a) Peptonization. 



(b) Reduction. 



(c) Alkaline. 



4. Glycerin Potato: 



(a) Growth — Moderate. 



(b) Dull creamy yellow growth. 



5. Gelatin Agar Colony: 



(a) Granular. 



(b) White. 



(c) Lacerate edges. 



III. Physical and Biochemical Features : 



1. Fermentation tubes, neutral red brotli plus 



(a) Dextrose: 



Gas — None. 

 Reaction — Acid. 

 Green iridescence. 



(b) Lactose: 



Gas — None. 

 Reaction — Alkaline. 



(c) Saccharose: 



Gas — None. 

 Reaction — Acid. 



2. Peas in distilled water: 



(a) Aerobic cultures : 



Gas. 



Very moist growth whicli develops a touch of 

 bright tan color. . 



(b) Anaerobic culture: 



Gas. 



3. Peptone Solution: 



(a) Indol production, 



4. Nitrate Peptone Solution: 



(a) Nitrate reduction. 



(b) Ammonia production. 



5. Starch Peptone Solution: 



(a) Starch reduction. 



(b) No test for sugar. 



G. Temperature Relations : 



(a) Grows readily both at 25 degrees and 37 degrees C. 



(b) Not killed by heating at 120 degrees C. for ten 



minutes. 



(c) Killed by heating at 120 degrees C. for twenty 



minutes. 



