EXPERIMENT STATION BULLETINS. 649 



Bacillus K. 

 Found in can A I 4. 



I. Morphology: 



1. Vegetative Cell : 



(a) Large rod, usually in short chains. 



(b) Size — 24.25 x 0.75 microns. 



. 2. Endospores : 



(a) Position — Central. 



3. Motility : 



(a) Slowly motile. 



4. Staining : 



(a) Gram negative. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics: 



1. Gelatin Stab: 



(a) Liquefaction — Crateriform. 



2. Nutrient Broth : 



(a) Top growth finely wrinkled. 



(b) Medium clear. 



3. Litmus milk : 



(a) Peptonization. 



(b) Reduction. 



(c) Alkaline. 



4. Glycerin Potato : 



(a) Growth — Moderate, contoured, raised. 



(b) Dry, chalky white growth, 



5. Gelatin Agar Colony: 



(a) Eegular. 



(b) White with thicker creamy spot in center. 



III. Physical and Biochemical Features: 



1. Fermentation tubes, neutral red broth plus 



(a) Dextrose: 



Gas — None. 

 Reaction — Acid. * 

 Iridescent. 



(b) Lactose: 



Gas — None. 

 Reaction — Alkaline. 



(c) Saccharose: 



Gas — None. 

 Reaction — Acid. 



