EXPERIMENT STATION BULLETINS. 651 



3. Litmus milk : 



(a) Keduction iu twenty-four hours. 



(b) Coagulation in thirty-six hours. 



(c) Eeaction — Acid. 



4. Glycerin Potato : 



(a) Growth vigorous. 



(b) Dull wrinkled whitish growth. 



5. Gelatin Agar Colony: 



(a) Tree-like spreading growth. 



(b) White in color. 



III. Physical and Biochemical Features: 



1. Fermentation tubes, neutral red broth plus 



(a) Dextrose: 



Gas — None. 

 Eeaction — Acid. 



(b) Lactose: 



Gas — None. 

 Reaction — Neutral. 



(c) Saccharose: 



Gas — None. 

 Reaction — Neutral. 



2. Peas in distilled water: 



(a) Aerobic culture: 



Gas. 



Moist growth with touch of bright tan color. 



(b) Anaerobic culture: 



Gas — None. 



White growth beneath surface of oil. 



3. I'eptone Solution : 



(a) Indol formation. 



4. Nitrate Peptone Solution : 



(a) Nitrate reduction. 



(b) Ammonia formation. 



5. Starch Peptone Solution : 



(a) Starch reduction. 



(b) No sugar. 



(c) Reaction — Acid. 



G. Temperature Relations: 



(a) Growth at both 25 degrees C. and 37 degrees C. 



(b) Not killed by heating at 120 degrees C. for ten 



minutes. 



(c) Killed by heating at 120 degrees 0. for twenty 



minutes. 



