652 STATE BOARD OF AGRICULTURE. 



Bacillus Z. 

 Found in can A V 13. 



I. Morphology: 



1. Vegetative Cell: 



(a) Slender rod. 



(b) Size — 1-2x0.5 microns. 



2. Endospores : 



(a) Produced subterminally. 



(b) Shape — Oval. 



3. Motility : 



(a) Actively motile. 



4. Staining: 



(a) Gram negative. 



(b) Stains with ordinary dyes. 



II. Cultural Characteristics: 



1. Gelatin Stab: 



( a ) Liquefaction — Inf undibulif orm. 



2. Nutrient Broth: 



(a) Top growth soft and heavy; breaks producing a 

 cloudy precipitate. 



3. Litmus milk : 



(a) Increased alkalinity. 



(b) No i)eptouization. curd or reduction. 



4. Glycerin Potato : 



(a) Potato gray. 



(b) Growth flat, yellowish gray. 



5. Gelatin Agar Colony: 



(a) Raised. 



(b) Glistening. 



(c) Yellowish white. 



III. Physical and Biochemical Features : 



1. Fermentation tubes, neutral red broth plus 



(a) Dextrose: 



Gas — None. 

 Reaction — Neutral. 



(b) Lactose: 



Gas — None. 

 Reaction — Neutral. 



(c) Saccharose: 



Gas — None. 

 Reaction — Neutral. 



