EXPERIMENT STATION BULLETINS. 655 



VII. B. prodigiosus. 



1. Aerobic Culture: 



(a) Gas production in forty-eiglit hours at 25 degrees 



C. and 37 degrees C. 



(b) Eed color produced throughout tube, reddest at 



top. 



2. Anaerobic Culture: 



(a) Gas production in 96 hours at 37 degrees C. No 



gas at 25 degrees C. 



(b) Growth throughout tube but no color. 



VIII. B. ruler. 



1, Aerobic Culture: 



(a) Gas production in twenty-four hours at 37 degrees 



C. 



(b) Gas in forty-eight hours at 25 degrees C. 



(c) Eed color prominent at 37 degrees C. Slight at 



25 degrees C. 



2. Anaerobic Culture: 



(a) Gas production in twenty-four hours at 37 degrees 



C. 



(b) No gas at 25 degrees C. 



(c) Slight red color beneath the oil. 



IX. B. aerogenes. 



1. Aerobic Culture: 



(a) Gas production in twenty-four hours at both 25 



degrees C. and 37 degrees C. in large volume. 



(b) Whitish growth throughout the tube. 



2. Anaerobic Culture: 



(a) Gas production in twenty-four hours at both 25 



degrees C. and 37 degrees C. in large quantities. 



(b) Whitish growth throughout the tube. 



X. B. viscosiis. 



1. Aerobic Culture: 



(k) Large volume of gas in twenty-four hours at both 



25 degrees C. and 37 degrees C. 

 (b) Decided cloudiness of liquid. 



2. Anaerobic Culture: 



(a) Large volume of gas in twenty-four hours both at 



25 degrees C. and 37 degrees C. 



(b) Cloudiness in liquid, otherwise little change of ap- 



pearance. 



