I.IG ANNUAL REPORT OF THE Off. Doc. 



REPORT OF THE RA("rEKIOr/)r. K'AI. LAP.ORATORY 



The i);uli(>l<»^i(al and iKK-tcrioloj^ical work iiuhidiiij:; the prepara- 

 tions of lulieiciilin, nialk'in, anthrax vaccine and the diagnosis of 

 all six'ciniuis received li;is heen (u.ne by Dr. Jolin Keichel, Chief of 

 ihe laboratory assisted hv Dr. Jolin H. Engle and Dr. Edward 

 Kecords. 



The folloAvinji' report is submitted by Dr. Keicliel, showing the 

 cliaracter and anioiint of work done by the laboratory during the 

 past year: 



The work of the Laboratory of the Pennsylvania State Livestock 

 Sanitary Uoard foi- tlie year 1010 may be Inielly summarized as fol- 

 lows: 



TUBP:RCULIN. L*SJ20 c. c. of concentrated tul»erculin have been 

 made requiring tlie inoculation of 2,2oo flasks, each containing 200 

 c. c, 19 flasks, eacli containing 1,000 c. c. an<l 22 Hasks, each contain- 

 ing 500 c. c. of neutral glycerin bouillon. The name strains of tu- 

 Itercle bacilli were usi'd as in 1000. Tubeiculin made from the 

 various ty};e of tubercle bacilli including hunum, bovine, and avian, 

 was kept separate and an attempt was made to send tuberculin pre- 

 pared with tubercle bacilli of the human type in the testing of cattle 

 for the first time. Where it was known that cattle were to be re- 

 tested or re-test tuberculin was requested, tul)erculin prepared with 

 tubercle bacilli of the bovine type was sent. Tuberculin made with 

 tubercle bacilli of the ovian type was used for experimental pur- 

 poses only. During the year, 41,0.'>0 doses of tuberculin, containing 

 0.4 c. c. of concentrated tuberculin, were sent out for the initial test 

 of cattle; 1,903 doses, containing 1.2 c. c. of concentrated tuberculin, 

 for re-testing; 54, containing more or less concentrated tuberculin 

 for special puri)oses. 



TUBERCrLOSIS A'ACCINES. Nearly 1,000 c. c. of vaccine were 

 prepared but not all used, in that some of the experimental work 

 was discontinued during the latter part of the year. 



MALLEIN. 900 c. c. of concentrated mallein have been made. 

 Of this, 1,425 doses, each containing 0.25 c. c. of concentrated mallein 

 were used in the initial test; 429 doses, each containing 1.2 c. c. of 

 concentrated mallein, for re-testing. 



ANTHRAX VACCINE. 1,379 doses of anthrax vaccine No. 1 and 

 No. 2 for cattle; 71 doses of No. 1 and No. 2 for horses, Avere sent out 

 from the laborator3\ In one instance it was advisable to follow an- 

 thrax vaccine No. 2 with anthrax vaccine No. 3, and 11 doses for 

 cattle and G doses for horses were sent out for that purpose. Reports 

 following vaccinations were uniformly good. 



AUTO- VACCINES. During the year, 61 specimens of pus were 

 received for the purpose of making a vaccine from the growth of 

 the bacteria in the pus. These specimens included material from 

 cases of pyogenic infecti(ms such as fistula of the witliers. pole evil, 

 quittor, al>scesses and infected wounds in liorses and mules. The 

 method of procedure followed in the c(dlecfing of a specimen and 

 the preparation of vaccine is as follows: 



The material is collected upon sterile cotton swabs in test tubes 

 supplied for that purpose. Upon their arrival at the Laboratory, 

 a small portion of the material is placed in a llask containing ap- 



