No 7. DEPARTMENT OF AGRICULTURE. 171 



that it was not necessary to take more than three temperature meas- 

 urements following the injection of the tuberculin and that these 

 measurements need not be taken after the sixteenth liour. It seems 

 to me that we have ample proof to show that this is a very erron- 

 eous way of applying a test as valuable as the tuberculin test, and 

 in this manner many animals have escaped. 



These two animals were removed from the milking herd and placed 

 in a barn some distance from the main stable and were attended by 

 a party who had no connection with the milking herd, but who at- 

 tended to a bull and a lot of calves which will be mentioned later. 

 They were kept separate and their milk pasteurized for a period of 

 eighty-two days, when they were again tuberculin tested with the 

 following results: 



TUBERCULIN TEST 

 Proceda. Francelmar. 



Preliminary temperatures. Preliminary temperatures. 



Feb. 10, '09. 



5 A. M. 102.4 102.4 



7 A. M. .102 102.1 



9 A. M. 99.4 101.2 



11A.M. 99.4 100.4 



1 P. M. 100.4 100.8 



3 P. M. 100.6 101. 



5 P. M. 102.2 101.4 



Eight P. M. Injected — Dosage, 1 drachm of solution for each ani- 

 mal, containing 1,200 milligram concentrated tuberculin. 



Temperatures following injection. Temperatures following injection. 



Feb. 11, '09. 



5 A. M. 102.6 100.6 



7 A.M. 102.4 101.4 



9 A. M. 100.6 101. 



11 A. M. 101.2 102. 



1 P. M. 103 102.2 



3 P.M. 101.2 101.4 



5 P. M. 103 101.6 



7 P. M. 102.6 



It will be noted that the one animal gave a temperature at 1 P. 

 M. of 103° and again at 5 P. M. of 103°, but the intermediate tem- 

 perature at 3 P. M. was normal and it was considered that the ir- 

 regularity in the temperature measurements was due to some other 

 cause rather than tuberculosis. It has not been the experience of 

 the writer that a tuberculous animal will give rise in temperature, a 

 fall and then another rise folloAving the injection of tuberculin un- 

 less there has been some local cause for the same, such as undue ex- 

 citement, drinking cold water, feeding, milking, etc. Following this 

 test, which was considered negative, these animals were returned 

 to the milking herd. They were again tested on April 27, 1909 and 

 May 4, 1910, at which time they both gave negative tests. 



