No. t. t>EPARTAlENT OJ^ AGtllCtlT.TTIRE. ifS 



It was not possible for the writer to be present at tlie autopsies, 

 though Dr. B. T. Woodward of the Bureau of Animal Industry and 

 Dr. W. L. Moss of John Hopkins conducted the same, and 1 have 

 the folloAving report from Dr. li. W. Hickman, Chief of the Quaran- 

 tine Division of the Bureau of Animal Industry: 



"Kef erring to cattle * * " * Avhich were slaughtered at the 

 Baltimore Butchers' Abattoir, Baltimore, Md., January 81st, and 

 post-mortem examinations by Dr. li. T. Woodward of this office to- 

 gether with your representative, you are advised that the cow Turby 

 immunized showed a supra-mammary gland the appearance of which 

 was suspicious of tuberculosis. This tissue was submitted to the 

 pathological division of the Bureau and two guinea pigs were inocu- 

 lated February 2nd. When examined at autopsy on March 7th, 

 both of these tests animals were found to be tuberculous. 



"The tubercle bacilli in both instances were similar in appearance 

 and were composed mainly of individuals of medium, long and in 

 some cases slightly curved formation, but experiments weie not 

 made to determine other characteristics which would justify their 

 definite classification into the human type. 



*' 'Masher's Kepose' immunized showed a suspicious supra-mammary 

 gland, but tubercle bacilli were not demonstrable microscopically or 

 through the inoculation of guinea pigs. 



" 'Proda's Sultana 2nd' immunized, showed hemorrhagic bronchial 

 glands. 



" 'Dewey's Ship,' immunized showed slight calcified areas in a 

 sub-lumbar gland. 



" 'All carcasses were passed for food." 



The animal Turby which showed no uiicroscopical lesions, though 

 the suspected material from the supra-mammary gland wlien injected 

 into guinea pigs Avas capable of producing generalized tuberculosis, 

 was given her first dose of tuberculosis vaccine March 7, 1909 and 

 four subsequent doses between the time mentioned and January 27, 

 1910. During this time she was allowed to mingle with the other 

 members of the herd which were believed not to be infected. How- 

 ever, the test of 1910 showed tliat the herd contained aft'ected ani- 

 mals, and it is probable that this particular animal may have be- 

 come infected during this time, inasmuch as it is known that an ani- 

 mal has less resistance for a short time (4 to 6 weeks) following the 

 vaccination than normal. 



In the case of the second immunized animal, Dewey's Ship, another 

 explanation can be offered. This animal was first vaccinated against 

 tuberculosis on January 30, 1905 and received three vaccinations end- 

 ing June 15th, 1905, and it is within reason to believe, with the knowl- 

 edge at hand, that the immunity conveyed by the vaccine had been 

 lost in this length of time. 



