No. 7. DEPARTMENT OF AGRICULTURE. 119 



of the horses rapidly improved, and diseased foci soon ceased to 

 discharge and liealed completely. The recovery appears to be 

 complete in every case, the scars from the previous local treatment, 

 lancing and point firing, being the only trace of the previous 

 lesions. The animals are still under observation, with check horses 

 which have not received treatment. 



In the latter part of June the laboratory was moved into the new 

 veterinary building at the University of Pennsylvania. The new 

 quarters are much better suited to the work than the former. The 

 following statement of the work within the laboratory is from a 

 report prepared by Dr. Reichel: 



During the year the following biological products were made and 

 entirely used up, leaving little or no stock for the year to come. 



Tuhcrculin. 15,145 c. c. of concentrated tuberculin have been 

 made, requiring the inoculation of 2,090 flasks, each containing 200 

 cubic centimeters of glycerine bouillon. It will be noted that a 

 larger number of flasks were inoculated and less tuberculin pro- 

 duced than last year. In the initial attempts to make tuberculin in 

 the new laboratory, contaminations of the newly inoculated flasks 

 were not infrequent and had to be discarded. This was reduced to 

 a very small percentage later in the year. 



Tuberculosis vaccine. Between 500 and 600 c. c. of vaccine have 

 been made each month. During the year it was found by experi- 

 ment that vaccine kept for three weeks at a low temperature would 

 lose in virulence, so that it would take twice as long to kill a guinea 

 pig as it did when it was first prepared. As a rule, from 50 to 100 

 c. c. were kept in a small rubber stoppered flask. As the dose varies 

 and only part of the vaccine in each flask is used it is impossible to 

 estimate how many doses were made. 



Mallein. 555 c. c. of concentrated mallein have been made, which, 

 with the balance left over from 1907 used early in the year, was 

 sufficient to supply the demand, leaving, however, no stock for the 

 year to come. 



Anthrax vaccine. 1,063 doses of vaccine No. 1 and No. 2 were 

 sejit out. All reports following A^accination were favorable. 



Specimens received for examination. During the year 799 speci- 

 mens were received at the laboratory for diagnosis, of which 318 

 were specimens of blood, 168 heads, 126 tissues, 102 of pus, 54 

 chickens, 11 of skin, 11 tumors, 3 of urine and 52 miscellaneous 

 specimens. Twenty-nine of these arrived in such a badly decom- 

 posed condition as to make an examination impossible. 



The specimens received included: 



Rabies. 182 heads were examined. Of these 20 were of experi- 

 mental rabbits used in the animal inoculation tests in connection 

 with the examination of doubtful cases during the year; 14 were 

 positive and 6 negative. Of the 163 heads received for examination 

 for rabies, 143 were dogs, of which 91 or 75 per cent, were positive, 

 31 or 25 per cent, negative; and in 21 or 14 per cent, decomposition 

 interfered with the examination; 7 horses, 4 positive, 1 negative, 2 

 badly decomposed; 11 cows, 6 positive, 4 negative, 1 decomposed; 

 2 deer, both positive; one cat positive; one sheep positive. 



The diagnosis was made in one of the three following ways: 



1. Smears and sections of the hippocampus major and of the 

 cerebellum were examined for Negri bodies. 



