No. 7. DEPARTMENT OF AGRICULTURE. 121 



Epizootic lymphangitis. The specimens received for examination 

 were more numerous early in the year. Oi: the 47 specimens, the 

 organism saccharomyces fa/rcminosus was demonstrated in 16, 

 In 15 the organism Avas not found. In 16 the specimens were 

 poor and it was impossible to make a satisfactory examination. 

 Tn most of these not enough material was collected, and in each 

 instance a request for auotiior specimen was made. 



Anthrax. 20 specimens were received for examination, including 

 10 from cattle, 2 from horses, 1 hog and 1 sheep. Of these the 

 anthrax bacterium was demonstrated in 6, 5 in cattle and 1 in the 

 hog. A rule was made in the laboratory tliat anthrax vaccine No. 1 

 be prepared whenever a specimen suspected of anthrax was 

 received, and when the diagnosis was established, the vaccine would 

 immediately be sent after learning the number of animals exposed. 

 This avoided much delaj' and incurred the earliest possible pre- 

 cautions. 



Hog cholera. Six specimens were received from animals sus- 

 pected of hog cholera, consisting in two instances of the entire hogs, 

 and in 4 of sections ol the internal organs. A positive diagnosis 

 was made in tv>'o from the characteristic intestinal lesions and 

 hemorrhagic kidneys, in 4 no lesions of hog cholera were found. 



Actinomycosis. The Cladothric actinomycosis was found in 4 

 specimens, 2 of which were sent to the laboratory for diagnosis and 

 two as museum specimens. The 2 specimens received for diagnosis 

 were both from animals slaughtered for beef. The lesions found 

 were small nodules the size of a pea, on the parietal peritoneum. 

 In both instances, the diagnosis was made upon microscopic ex- 

 aminations of prepared sections. 



Chronic bacterial dysentery. Eleven specimens were received 

 consisting of scrappings and faeces from supposedly infected cattle. 

 The acid fast organism w^as demonstrated in 8 and not demonstrated 

 in 3. In addition, fresh specimens showing the characteristic 

 reddened areas of the intestinal mucous membrane were received 

 on three separate occasions for museum specimens. 



Foot-and-mouth disease. No fresh specimens were sent to the 

 laboratory, but muzzles, udders and feet of the cattle and legs 

 showing lesions in all stages of the disease were collected, at the 

 time the animals Avere destroyed, and immediatelj' placed in pre- 

 serving fluid. These specimens were permitted to remain on the 

 infected premises for several weelis, until after the entire place had 

 been cleaned up and disinfected. The preserving fluid was changed 

 once in this time. As the preserving fluid is a strong disinfectant 

 and readily destroys the foot-and-mouth disease virus, it Avas not 

 considered dangerous to transfer these to the laboratory. 



Milk. SeA'en specimens of milk were received. In 5 a bacterial 

 count was requested, which was made. Two were from cows with 

 inflamed udders and in both streptococci were found. In addition 

 to these 7 samples of milk, many samples of wiiich no exact number 

 can be given, used in the experimental w^ork carried out by Dr. 

 Henry C. Cam])bell, Avere received from botli the experimental farm 

 and milk depots of Philadelphia and other cities in PennsylTania. 

 Much work was done by Dr. Campbell in determining the number and 

 in identifying the bacteria in milk from normal and inflamed cows' 

 udders. All known methods of determining leucocytes in milk were 



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