122 ANNUAL, REPORT OF THE Off. Doc. 



carried out upon a large number of samples and the change in the 

 number of leucocytes in samples of milk heated to various degrees 

 was thoroughly investigated. This work was done in co-operation 

 with the Pathological Division of the United States Bureau of 

 Animal Industry. The work is now ready for publication. 



Pigeons. Six pigeons were received. Five were from a Philadel- 

 phia Hock of several hundred. All 5 showed nodules in the wings or 

 legs, which, upon examination, were found to be lesions of tuber- 

 culosis. Some experimental work was done with this avian tuber- 

 culosis. Lesions were produced in several rabbits inoculated with 

 the tuberculous material from the pigeons, but attempts to infect 

 4 healthy pigeons with the lesions produced in rabbits were not 

 successful. 



Poultry. Fifty-four fowls were received, including 45 chickens, 

 4 guinea hens, 3 turkeys and 2 duck.s. Of these 28 were received 

 from a farm near Valley Forge, Pa. A turkey and 2 guinea hens 

 were first received early in February with a history that 27 head 

 including 7 turkeys, several guinea fowls and the remainder 

 chickens were found dead, lying under the perches of the poultry 

 bous(\ As soon as the turkey and two guinea hens arrived at the 

 laboratory, complete autopsies were made and in each district liver 

 lesions were found. Cultures were made from the internal organs 

 of all three fowls and a Gram negative diplococcus was isolated in 

 most of the cultures made. This organism was highly virulent for 

 guinea pigs, rabbits, goats and chicks, which were infected and 

 death produced in less than 24 hours, following the feeding of or 

 infection with the organism. This culture was studied but not 

 identified within the year. 



The remainder of the flock, 25 in number, were received late in 

 February and kept under observation for some time without show^- 

 ing any symptoms; several, however, were chloroformed and cultures 

 made, but no lesions were found and the cultures remained sterile. 



There remained but little doubt that the diplococcus isolated 

 from the first three fowls was the cause of death of the larger por- 

 tion of the flock. Tbe infection was a highly acute one, a form 

 of apoplectic septicaemia. 



Of the other fowls received during the year a diagnosis of tuber- 

 culosis was made in a turke3% 3 chickens suspected of being 

 poisoned were received and in four instances chickens were returned 

 to the owners after having been under observation for a long period 

 at the laboratory without showing any symptons. 



Miscellaneous specimens. These included tissues from a calf 

 showing Melanosis; tissues of calf suspected of hemorrhagic sep- 

 ticaemia; the heads of 2 sheep showing the oestrus ovis in the 

 sinuses; parasites, gastrophilus equi, trichina spiralis, strcugylus 

 contortus and cysticerous pisiformis; one hog's head, showing 

 rickets, sample of short hay and hay dust which was found to 

 contain considerable ergot; tumors, including fibrona, round cell 

 sarcoma, h^ypernephroma or gravity tumor, melanotic sarcoma and 

 carcinoma. 



HOG CHOLERA. Reports of this disease were received from the 

 counties of Berks, Butler, Centre, Columbia, Cumberland, Dela- 

 ware, Franklin, Indiana, Lancaster, Lawrence, Lebanon, Luzerne, 

 Lycoming, McKean, Montgomery, Monroe, Northumberland, Schuyl- 

 kill, Sullivan, Venango, Washington, Westmoreland, Wyoming and 



