EXPERIMENT STATION REPORTS. 241 



Congo red agar slant: 24 hr., no noticeable growth. 12 da., scarcely 

 visible growth along needle tract. 



Litmus lactose bile agar slant: 24 hr. growth doubtful if any, 12 da., 

 less growth than on Congo red agar. 



Pork Dextrose broth: 0.5; 24 hr. no growth. 12 da., no growth. 



Potato slant: 24 hr. doubtful if any growth. 12 da., moist growth 

 along path of needle, colorless. 



Corn agar slant: 24 hr. growth spreading and abundant. Small white 

 dots in the abundant growth seem to be typical of this organism, these 

 colonies being confluent and not distinct. Growth pale white and nearly 

 translucent. Condensation water is cloudy, white. 12 da., abundant 

 growth, translucent, marmorated, slightly opalescent, moist, consider- 

 able sediment at bottom of condensation water. 



Lactose broth fermentation tube: 24 hr. no noticeable growth. 



Dextrose broth fermentation tube: 24 hr., no noticeable growth. 12 

 da., reinoculated, no growth. 



Saccharose broth fermentation tube: 24 hr. no noticeable growth. 12 

 da., a macaroni-like growth in the long arm and dense growth in bulb. 



Sour whey agar slant: 24 hr., growth slight if any. 12 da., growth at 

 base of slant scarcely visible. 



Litmus milk: 24 hr., reduction at surface. 12 da., quite strongly acid 

 not curded. 



Dunham's solution: 24 hr., no growth, 12 da., sediment which comes 

 up with a swirl, no clouding. 



Nitrate peptone solution: 24 hr., no growth. 12 da., no growth. 



Esculin bile solution: 24 hr., no growth. 12 da., no growth. 



Corn tube: 24 hr., gas formation in bottom of tube. 12 da., gas for- 

 mation very pronounced. 



Peas {tube) anaerobic {oil): 24 hr., gas formation abundant and con- 

 tinues vigorously. 



String bean tube, anaerobic {oil): 24 hr., clouding of liquid in tube, 

 small amount of gas. 



Dextrose gelatin agar slant — 0.5: 24 hr., growth like streptococcus. 

 12 da., colonies much smaller than on ordinary dextrose agar; a small 

 amount of sediment in condensation water. 



Gelatin stab: 24 hr., no growth. 12 da., no growth. 



"This bacillus is an example of the type of organisms very frequently 

 found in spoiling canned foods, i. e. an organism difficult to isolate on 

 account of its peculiar cultural requirements. This organism grows well 

 on no medium used except corn agar and sterile corn and peas. This 

 peculiarity of certain organisms is very frequently the explanation why 

 certain spoiled canned foods are declared sterile or the organisms isolated 

 do not show gas production in ordinary media." 



"A sample of sausage which had been feeding experiments indicated 

 that it was not poisonous at the time and contained no toxin-producing 

 organisms." 



