PREFACE 



This manual was written in response to a need for an outline of instruction 

 in the technique of microdissection and microinjection. All directions have teen 

 made especially simple and detailed so that students who have no recourse to personal 

 instruction may find it easy to learn the technique. In preliminary form, the manual 

 has been used for the past two years at Washington Square College, and at Woods Hole. 



Although it deals chiefly with the Chambers apparatus, the principles 

 described are fundamental, and may be applied, with suitable minor modifications, 

 to any other type of micromanipulative apparatus now in general use. 



It is felt that the bibliography is an important component of the manual, 

 since the scope of this book permits us to deal but briefly with special methods 

 and techniques. Investigators interested in greater elaboration of any special 

 problem will find it necessary to refer to original publications. The bibliography 

 also serves to familiarize the reader with the range of application of the micrurg- 

 ical method. 



We are greatly indebted to Miss Helen Holt for invaluable aid in assembling 

 the manuscript and in compiling the bibliography. Our thanks are due to Mr. C. G. 

 Grand for aid in preparation of the drawings, many of which are original, and 

 others redrawn from various sources. We also desire to express to E. Leitz our 

 appreciation of their kindness in contributing the photograph used as the frontis- 

 piece. 



Ruth B. Howland 

 Morris Belkin 



Department of Biology 

 Washington Square College 

 New York University 

 New York, February 27, 1931. 



