placing a 100 Watt Mazda C spotlight bulb, B, within a light shed made of metal, 

 ,Sh. The bulb should be placed in a horizontal position and attached to a socket, 

 Sk, which can be rotated so that the two large filaments within the bulb face 

 down. This is done to avoid overheating and melting of the large filaments of 

 the bulb. To avoid either displacing the bulb or socket, a switch, Sw, is inter- 

 posed in the circuit. The shed is made of galvanized iron with a window cut out 

 in one face — the "front" face. Metal prongs are soldered around the two sides 

 and lower edge of the window to form a slot into which may be inserted a 3" x 3" 

 daylight glass. The "back" edge of the roof should be open to permit ventilation. 

 An arched opening is cut at the base of one side (either right or left) to ac- 

 commodate the light socket. 



TV* 3 



To absorb the heat of the lamp and concentrate the light on the mirror of the 

 microscope a 500 cc Florence flask filled with distilled water should be placed 

 between the microscope mirror and the source of light. A convenient distance is 

 about three xnches from the mirror. The light shed may be placed about 18 inches 

 from the mirror. 



Assembling of Complete Apparatus for Microdissection 



Place the heavy metal base on your table so that the pillar sockets are away from 

 you. Pick up right and left instruments and insert the pillars in these holders 

 in such a position that the bars form the two sides of a triangle with the open 

 base toward you. Clamp the instruments loosely in place by means of the screws in 

 the side of the sockets. Arrange their positions so that the coarse adjustment 

 screws are clear. Place the microscope on the base and push it CAREFULLY forward 

 so that the stage and its attachments easily clear the bars. If any part of the 

 microscope sub-stage apparatus comes in contact with the bars, the latter must be 

 moved outward slightly or the entire instrument must be lowered in the pillar 

 socket just enough so that all parts of the microscope are free from contact. The 

 instruments may now be firmly clamped in place. Continue moving the microscope 

 forward^ until the ends of its U-shaped base are firmly in contact with the two 

 metal wings which are screwed into the manipulator base at the foot of the pillar 

 sockets. When the above manipulations have been successfully completed, fasten 

 the microscope firmly into place by means of the third metal wing found at the 

 rear ol the base plate (the end nearest you). It may be necessary to ad.iust this 



