10 



1. Schaeffer, A. A. 1920 



2. Mast, S.O. 1926 



3. " " 1928 



4. Folger, Harry T. 1926 



5. Hopkins, D.L. 1928 



6. " " 1929 



7. Calkins, G.N. 1911 



8. Howland, R.B. 1924 



Bibliography 



Amoeboid Movement. (Note table on A. proteus, 



A. discoides and A. dubia) . 



Structure, Movement, Locomotion and Stimulation 



in Amoeba, J. Morph. and Physiol., 41, 347. 



(Read bibliography of this paper). 



Factors involved in change of form in Amoeba. 



J. Exp. Zool., 51, 97. 



Effects of Mechanical Shock on Locomotion in 



Amoeba proteus, J. Morph. and Physiol., 42, 359 



The effect of certain physical and chemical 



factors on locomotion and other life processes 



in A. proteus, J. Morph. and Physiol., 45, 97. 



The effects of the substratum, divalent and 



monovalent cations on locomotion in Amoeba 



proteus. J. Morph. and Physiol., 48, 571. 



Effects produced by cutting Paramecium cells. 



Biol. Bull. 21, 36. 



Dissection of the pellicle of Amoeba verrucosa. 



Jour. Exp. Zool., 40, 263. 



MICRODISSECTION EXPERIMENTS 

 I. On Amoebae. 



A. Amoeba proteus . 1. Make a small hanging drop on a cover slip containing a half 

 dozen amoebae.* Bring up one needle and transfix an organism against the cover sli] 

 Bring up the other needle, pierce the animal. Lower both needles. Describe the ef- 

 fect of puncture on the amoebae. 



2. Pierce another amoeba with two needles as above. Tear the 

 amoeba by moving one needle laterally. Describe the effect. 



3. Without injuring it, move an amoeba to the edge of the droj? 

 with one needle. Hold the amoeba against the cover slip until it attaches itself 

 and begins to crawl. Then press the other needle firmly against the amoeba, and by 

 moving the needle backwards and forwards, cut the cell against the cover slip. Cut- 

 ting may sometimes be accomplished by using only the point of the microneedle, 

 though usually it is more easily done by raising the needle until the tip is flat- 

 tened against the cell. 



4. Dissect out a nucleus and impale it on a needle point. 



B. Amoeba dubia . Repeat the above. Note the specific differences in these two 



organisms as to: 



a. relative ease of puncturing wall. 



b. relative ease of severing in two, etc. 



c. variation in form, consistency, or reaction of nuclei. 



♦ Footnote : A simple transfer pipette may be made, and the amoeba handled by one of 

 the following methods: (1) Mouth Pipette : Draw out a five inch piece of clean glass 

 tubing over a Bunsen burner and point it off. Attach to the other end six inches of 

 soft rubber tubing. Practice drawing up several amoebae in a small drop of culture 

 medium and depositing them on the cover slip. 



(2) Hand Pipette : Draw to a fine point a three inch length 

 of clean glass tubing of small diameter (3 mm). Attach to the large end two inches 

 of soft rubber tubing of about the same bore. Fold back on itself about half an inch 

 of this tubing and tie it securely in this position with a bit of string. With this 

 small hand pipette practice picking up amoebae as before. 



