CHAPTER II. 

 GENERAL FORMULAE 



NOTE: Use distilled water in all solutions. 



ADAMKIEWICZ REAGENT: (for proteins). Concentrated sulfuric acid 

 1 volume, glacial acetic acid 2 volumes. Heat substance with this 

 reagent. Reddish- violet color denotes proteins. 



AGAR: 2% may be used to solidify various solutions. 



ALCOHOL, ACID: 70% alcohol 99 ml., cone, hydroclilonc acid 1 ml. 



ALCOHOL, ALKALINE: 70% alcohol 99 ml., 0.02 grams sodium bicarbonate, 



AMANN'S LACTOPHENOL: Phenol 20 grams, lactic acid (1.21 sp.gr.) 

 16.5 ml., glycerine 32 ml., water 20 ml. For herbarium specimens, 

 soften first in 1:10 lactophenol and then to pure lactophenol. For 

 algae use, lactophenol 5 ml., water 95 ml., cupric chloride and cupric 

 acetate each 0.2 grams. For mounting fungi add to the lactophenol 

 0.05 grams cotton blue. 



AMMONIACL\L SILVER SOLUTION: Dissolve 13 g. of silver nitrate in 

 about 250 ml. of water, add enough cone, ammonium hydroxide to re- 

 dissolve the precipitate whicli forms upon the first addition of the 

 NH^OH and make the volume up to 500 ml. with water. 



ANILINE WATER: Shake 4 ml, of aniline in 90 ml. of distilled water 

 for at least 15 minutes. Filter through a wet filter. Enough alcohol 

 may De added to make it 20% alcohol, if a weakly alcoholic solution is 

 des ired . 



BARFOED'S SOLUTION: (test for dextrose in presence of maltose) 

 13.3 g. cupric acetate in 200 ml. water, and 5 ml. of 38% acetic acid. 



BELAR'S SOLUTION: Water 100 ml., sodium chloride 0.9 g., potassium 

 chloride 0.02 g., calcium chloride 0.02 g. , sodium bicarbonate 0.02 g. 

 To this 0.25 to 0.5 g. glucose may be added. 



BENECHE'S NUTRIENT SOLUTION: ( Algae-bluegreen ) Water 1 liter, ammo- 

 nium nitrate 0.2 g., calcium chloride 0.1 g., potassium phospliate 

 (dibasic) 0.1 g., magnesium sulfate 0.1 g., 1% ferric chloride 1 drop. 

 For use dilute 2-9 times as required. (Courtesy Dr. Navez) 



