ammonium nitrate 1 g., potassium nitrate 2 g., sodium nitrate 2 g., 

 potassium bromide 0.2 g., potassium iodide 0.1 g. B. * Water 80 ml., 

 sodium phosphate 4 g., calcium chloride (anhyd) 4 g., cone, hydrochloric 

 acid 2 ml., ferric chloride 2 g. 



•Make as follows: To the sodium phosphate dissolved in 40 ml. of 

 water are added, first 2 ml. of HCl , then 2 grams of ferric chloride and 

 then 4 grams of calcium chloride dissolved in 40 ml. of water, taking 

 care to shake the mixture. There will be a brown precipitate of ferric 

 oxide on adding the last solution. It should be separated from the 

 liquid before using. Forty drops of solution A and 10 - 20 drops of 

 solution B are added to each 1000 ml. of sea water sterilized by keeping 

 at 70 C for about 20 minutes. 



MIQUEL'S SOLUTION: II (May be added to A and B) Sodium silicate 

 •9 HgO 5 g. , water 100 ml. 



MIQUEL'S SOLUTION: III (Allen's modification) A. Water 100 ml., po- 

 tassium nitrate 20.2 g. B. Water 80 ml., dibasic sodium phosphate 

 4 g. , CaCl2»6 H2O 4 g. , ferric chloride (melted) 2 ml., cone. HCl 2 ml. 

 For use, add 2 ml. of solution A and 1 ml. of solution B to each 1000 

 ml. of sea water. 



MOORE'S SOLUTION: Water 1000 ml., ammonium nitrate 0.5 g. , monobasic 

 potassium phosphate 0.2 g. , magnesium sulfate 0.2 g. , calcium chloride 

 (anhyd) 0.1 g. , ferric sulfate trace. 



NAEGELI'S SOLUTION: (culture medium for fungi) Water 1000 ml., di- 

 basic potassium phosphate 1 g. , magnesium sulfate 0.2 g., calcium 

 chloride (anhyd) 0.1 g., ammonium tartrate 10 g. 



NESSLER'S REAGENT: (Block and Benedict from Hawk and Bergein) 

 Mercuric iodide 100 g., potassium iodide 70 g. , sodium hydroxide 100 g. 

 Place 100 g. mercuric iodide and 70 g. of potassium iodide in a liter 

 volumetric flask and add about 400 ml. of water. Rotate until solution 

 is complete. Now dissolve 100 g. of sodium hydroxide in about 500 ml. 

 of water, cool thoroughly and add with constant shaking to the mixture 

 in the flask, and make up with water to the liter mark. If a precip- 

 itate forms, decant the supernatant liquid and use. 



PASTEUR'S SOLUTION: Potassium phosphate 2 g., calcium phosphate 0.2 g., 

 magnesium sulfate 0.2 g., ammonium tartrate 10 g., cane sugar (sucrose) 

 150 g., water to make 1 liter. 



PHENOL: To liquify add 10 ml. of water to each 450 g. melted phenol. 

 Melt by immersing bottle in hot water. 



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