CHAPTER III. 



KILLING AND FIXING FLUIDS 



COPPER ACETATE FORMALIN: Saturated cupric acetate in 40% formalde- 

 hyde. Dilute to about 4% for preservation of green algae. 



CUPRIC-PARANITROPHENOL FLUID: (Petrunkevitch) 60% alcohol 100 ml . , 

 nitric acid 3 ml., ether 5 ml., cupric nitrate (3 HpO) 2 g. , para- 

 nitrophenol cryst. 5 g. One part formaldehyde may be added to 4-7 

 parts of the fluid just before use. 



CUPRIC PHENOL FLUID: (Petrunkevitch) _A^: distilled water 100 ml., 

 nitric acid 12 ml., cupric nitrate (3 HgO) 8 g. _Bj 80% alcohol 100 

 ml., phenol cryst. 4 g. , ether 6 ml. Use 1 part of j\^ to 3 of B. 

 Will not keep after mixing. 



FAA: (General Biological Supply House) 50% alcohol 100 ml., 40% 

 formaldehyde 6.5 ml., glacial acetic acid 2.5 ml. 



HOLLANDE'S FLUID: Formalin sat. with picric acid 12 ml., absolute 

 alcohol 54 ml., benzene 3 ml., and nitric acid 1 ml. 



LANE'S SOLUTION: Potassium bichromate 2.5 g., mercuric chloride 

 5.0 g. , water 100 ml. 



NAVASCHIN'S FLUID: 10% chromic acid, 1.5 ml., glacial acetic acid 

 1 ml., formaldehyde (40%) 0.83 ml., water 32.67 ml. 



PRESERVING FLUID FOR GREEN ALGAE: Potassium chrom alum 10 g.j form- 

 aldehyde 5 ml., water 500 ml. 



STOCKARD'S SOLUTION: Water 85 ml., formalin 5 ml., glacial acetic 

 acid 4 ml., glycerine 6 ml. 



SUSA'S FLUID: Water 80 ml., mercuric chloride 4.5 g., sodium chloride 

 0.5 g., trichloracetic acid 2.0 g., formalin 20 ml., glacial acetic 

 acid 4 ml. 



WORCESTER'S FLUID: 10% formalin saturated with mercuric chloride, 

 90 ml., glacial acetic acid, 10 ml. 



OSMIUM AND PLATINUM CONTAINING FLUIDS: 



Osmium and platinum fixatives are costly and often do not keep 



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