14 ml., acid fuchsin (sat. aq. sol.) 7 ml., water 15 ml., absolute 

 alcohol 25 ml., methyl green (sat. aq. sol.) 12 ml., glycerin 10 ml. 

 Each solution should be thoroughly saturated (several days). Add the 

 ingredients in order named, shaking mixture well after each addition. 



FEULGEN'S REAGENTS: eg. nucleal reaction. 



GRAM'S STAIN: See Gential violet and Gram's iodine Chapter II. 



HEMALUM: (Mann's) liaematein 0.5 to 2 grams, absolute alcohol 100 ml., 

 glycerine 100 ml., water 100 ml., potassium aluminum sulfate (potassium 

 alum) 10 g. , glacial acetic acid 10 ml. Dissolve the dye in the acid, 

 add the alcohol, glycerine and 75 ml. of the water; then dissolve the 

 alum in the rest of the water with gentle heat and add to the other 

 ingredients. Five grams of aluminum sulfate may be substituted for the 

 potassium alum. 



HEMALUM: (Mayer's) Hematoxylin 1 g. , water 1 liter. Dissolve and 

 add sodium iodate 0.2 g. , ammonium aluminum sulfate or potassium alumi- 

 num sulfate 50 g. Heat to dissolve. When cool, filter. 



HEMALUM, ACID: (Mayer's) To Mayer's hemalum add glacial acetic acid 

 to 2%. 



HEMATOXYLIN STOCK SOLUTION: May be made by dissolving 1 part of hem- 

 atoxylin crystals in 10 parts of absolute alcohol. In the course of 

 several months or a year, this solution ripens to a dark wine-red color. 

 It may be used in making up the various hematoxylin solutions, and being 

 ripe, will stain at once. 



HEMATOXYLIN: (Delaf ield' s ) Aluminum ammonium sulfate (ammonium alum) 

 saturated aqueous solution 100 ml. Dissolve 1 g. of hematoxylin crystals 

 in 10 ml. of absolute alcohol, and slowly add it to the ammonium alum. 

 Expose to air and light for several weeks to 'ripen'. Ripening may be 

 accomplished at once with some degree of success through the addition of 

 a few ml. of hydrogen peroxide. When ripe, filter the solution and add 

 25 ml. of glycerine and 25 ml. of methyl alcohol. (Cf. Hematoxylin stock 

 solution ) . 



HEMATOXYLIN: (Erlich's acid) Hematoxylin 2 g., absolute alcohol 100 ml., 

 glacial acetic acid 10 ml., glycerine 100 ml., water 100 ml., aluminum 

 ammonium sulfate or aluminum potassium sulfate 10 g. Let the mixture 

 ripen in the light and air until it acquires a dark red color. 



-18- 



