m 



HEMATOXYLIN: (Galigher's) Hematoxylin crystals 0.5 g., aluminum 

 ammonium sulfate 0.3 g., 50% alcohol 100 ml., red mercuric oxide 0.6 g. 

 Dissolve hematoxylin and alum in the alcohol. When boiling add mercuric 

 oxide and boil for 20 minutes in a covered vessel. Let stand overnight. 

 Filter. Solution can be used at once. 



HEMATOXYLIN: (Harris's) Dissolve 20 g. of ammonium or potassium alu- 

 minum sulfate in 200 ml. of water with the aid of heat. Dissolve 1 g. 

 of hematoxylin crystals in 10 ml. of absolute alcohol and add to the war 

 alum solution. Bring rapidly to boil and add 0.5 g. of red mercuric oxide 

 When the solution turns dark purple, cool, and add 8 ml. of glacial acetic 

 acid. 



HEMATOXYLIN: (Heidenhain' s ) (iron-hematoxylin) _A. Ammonium ferric 

 sulfate (ferric alum) 2.5 g., water 100 ml. _B. 10% Hematoxylin stock 

 solution 5 ml., distilled water 100 ml. 



HEMATOXYLIN: (Mann's) M&ke up Erlich's Hematoxylin stain with haem- 

 atein instead of hematoxylin. 



HEMATOXYLIN: (Mayer's) Hematoxylin stock solution 10 ml., sodium 

 iodate 0.26 g., chloral hydrate 6.6 g., aluminum ammonium or aluminum 

 potassium sulfate 1.2 g. , water to 2 liters. 



HEMATOXYLIN: (Regaud's) Hematoxylin stock solution 10 ml., glycerine 

 10 ml. , water 80 ml. 



INDIGO- CARMINE: Indigo-carmine 0.2 g., water 100 ml. 



LOEFFLER'S METHYLENE BLUE: Methylene blue 0.3 g. , 95% ethyl alcohol 

 30 ml., 0.01% potassium hydroxide 100 ml. 



MALLORY'S TRIPLE STAIN: (for connective tissue) _\^ Acid fuchsin 0.5 g, 

 water 100 ml. _B. Anilin blue (Gruebler's water soluble) 0.5 g. , Orange G 

 (Gruebler) 2 g. , 1% aqueous phosphomolybdic acid 100 ml. 



MALLORY'S SOLUTION A': (also called Heidenhain' s azan stain) 0.5% 

 Azo-carmine with 5-10 drops of glacial acetic acid to each 100 ml. of 

 solution. Do not filter. Cf. also Azo-carmine. (Courtesy of Mrs. N. 

 Jones ) 



MUCI-CARMINE: (Mayer) Water 2 ml., carmine 1 g. , aluminum chloride 

 0.5 g., 50% alcohol 100 ml. Mix in order given, heat gently until the 

 fluid darkens (about 2 minutes); filter after 24 hours. To use, dilute 

 with 5 to 10 volumes of water. 



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