95% alcohol and boil, stirring constantly. Remove the paper from the 

 solution, filter, and add alcohol to make the solution up to 200 ml. 

 Add 4 ml. of cone, hydrochloric acid. The solution keeps well for 

 months . 



WRIGHT'S STAIN: Wright's stain (dry powder) 0.2 g., methyl alcohol 

 (absolute, neutral, acetone free) 60 ml. Filter after standing for 

 24 hours. 



STAIN SOLUBILITIES 



References : Conn, Biological Stains, 1946; Holmes, Stain Technology, 



1929 



Dye solubilities at 26*'C listed as grams of anhydrous dye per 100 ml. 



of saturated solution selected from the above references and printed 



with the permission of Dr. H. J. Conn. 



Aniline dyes are commonly used as saturated solutions, aqueous, or 

 alcoholic, unless other concentrations are given. 



KEY TO BIOLOGICAL USE OF THE STAINS: 



B - bulk N - nuclear 



C - cytoplasmic P - perfusion 



F - fat V - vital 



I - indicator 



Synonyms of the dyes are given in parentheses. 



Color, 95% Strength Use 



Index Name of Dye Water ,, , , , . 



Alcohol solution 



Number 



used 



1027 Alizarin Nil 0.125 I.C.V. 



1034 Alizarin red S (Alizarin red, water 



soluble; Alizarin carmine) 7.69 0.15 N.V. 



40 Alizarol orange G (Alizarin yellow 

 R; Mordant yellow PN; Orange R; 

 Anthracene yellow RN; Alizarin 

 orange) 0.40 0.57 



36 Alizarin yellow GW (Alizarin yellow 

 GG; Anthracene yellow GG; Mordant 

 yellow 2GT) 25.84 0.04 



184 Amaranth (Naphthol red S,C or 0; 

 Fast red; Bordeaux; Bordeaux 

 SF; Victoria rubin 0; Azo rubin; 



Wool red) 7.20 0.01 C. 



847 Amethyst violet (Heliotrope B; 



Iris violet) 3.12 3.66 N. 



■21- 



