ACETATE BUFFERS 



pH 3.6 - 5.6 



Reference: Recalculated from Walpole, J. S. , J. Chem. Soc. 



105 2501 (1914) 

 Stock Solutions: l.OOON Acetic Acid 



l.OOON Sodium Hydroxide NaOH 

 Both of these solutions are prepared according to the section on 

 standard solutions ( CHAPTER V ). 



Buffers: 



To make 1 liter of O.IM Acetate Buffer, pipette 100 ml. of the l.OOON 

 Acetic Acid stock solution into a 1 liter volumetric flask, burette in the 

 specified amount of l.OOON NaOH, and dilute to the mark with distilled water. 



PHOSPHATE BUFFERS 



pH 5.8 - 8.0 



Reference: Recalculated from Clark and Lubs; J. Biol. Chem. 

 ^ 479 (1916) 



Stock Solution: l.OOOM Potassium Phosphate, Monobasic 



(Potassium Dihydrogen Phosphate KH2P0«) 



Prepared by dissolving 136.14 g. of KHpPO^ in enough distilled water 

 to make 1 liter of solution. Merck's anhydrous reagent may be used directly 

 if freshly opened in the dry room and kept there. For greater accuracy, dry 

 for two hours at 120 C and place in a desiccator. The solid is conveniently 

 weighed in 25 - 30 g. lots, using glazed weighing paper. 



Buffers : 



To make 1 liter of O.IM Phosphate Buffer pipette 100 ml. of the l.OOOM 

 Phosphate stock solution into a 1 liter volumetric flask, add the volume of 

 l.OOON NaOH specified below, and dilute to the mark with distilled water. 



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