CONTENTS 



PREFACE 3 



ACKNOWLEDGMENT 4 



I. DYES FIRST USED 7 



II. CHEMICAL AND PHYSICAL PROPERTIES OF 



DYES 9 



Color Precipitates 



Ferric ferrocyanide 



Ferric tannate 



Hematein 

 Reversible Actions 

 Oxidation 

 Reduction 

 Physical Properties 



III. GENERAL USES OF BIOLOGICAL STAINS 13 



Acid Dyes 

 Direct Dyes 

 Mordant Dyes 

 'Basic Dyes 

 Nomenclature of Dyes 



IV. STAINING WITH CONTRAST DYES 18 



Selecting a Combination of Stains 

 Combinations of Two Stains 



Crocein Scarlet MOO and Niagara Sky Blue 

 Two Dyes Used Separately 

 Two Dye-solutions Mixed 

 Sun Yellow and New Methylene Blue 

 Rhodamine 6G and Aniline Blue 

 Combinations of Three Stains 

 Spot Test Using Basic Dyes 

 Time Test Using Acid Dyes 

 Combinations of Four Stains 



V. BIOLOGICAL STAINS 25 



A. Synthetic Organic Dyes 



C. I. Nos. 



Class I. Nitroso Colouring Matters 5 



Class II. Nitro Colouring Matters 7-10 



Class III. Monoazo Colouring Matters 16-211 



Class IV. Disazo Colouring Matters 248-520 



Class V. Trisazo Colouring Matters 581-594 



Class VI. Tetrakisazo Colouring Matters ... — 



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